ID A0A101CZH1_9RHOB Unreviewed; 820 AA.
AC A0A101CZH1;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Penicillin acylase {ECO:0000313|EMBL:KUJ86079.1};
GN ORFNames=AVO45_03705 {ECO:0000313|EMBL:KUJ86079.1};
OS Ruegeria marisrubri.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Ruegeria.
OX NCBI_TaxID=1685379 {ECO:0000313|EMBL:KUJ86079.1, ECO:0000313|Proteomes:UP000053791};
RN [1] {ECO:0000313|EMBL:KUJ86079.1, ECO:0000313|Proteomes:UP000053791}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ZGT118 {ECO:0000313|EMBL:KUJ86079.1,
RC ECO:0000313|Proteomes:UP000053791};
RA Shamseldin A., Moawad H., Abd El-Rahim W.M., Sadowsky M.J.;
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUJ86079.1}.
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DR EMBL; LQBQ01000001; KUJ86079.1; -; Genomic_DNA.
DR RefSeq; WP_068344530.1; NZ_LQBQ01000001.1.
DR AlphaFoldDB; A0A101CZH1; -.
DR STRING; 1685379.AVO45_03705; -.
DR OrthoDB; 9760084at2; -.
DR Proteomes; UP000053791; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000053791};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT TRANSMEM 7..30
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT ACT_SITE 262
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 195
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 334
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 337
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 820 AA; 90686 MW; 46D9875303562FC2 CRC64;
MSLLFRWLLR IAAGLVAMVV AAVALVYFLA SQSLPDYDQT LEVDGLSAPV EIVRDNANVP
HIFGASDPDV FFGLGYAHAQ DRLWQMTMLR RTVQGRLSEV FGKRTVTVDK LLRRLDLYRL
ALQSVEAQDD YTKAALKAYA AGVNARLAEV NEEALGRGAP EMFLFNAPVA PWQPGDSIAI
IKLMGLQLSG HLEEEVLRAR TSLMLEDPAR LSDILPDDPG SGIAALPEYA SLFPDLPRYA
ETIPMPEDPL SPFPRRGLAG ASNAWTAAPS RSASGGTLLA NDPHMGFSAP AAWYLARLEL
ANGGVIGASI PGVPTILTGR SDRLGWGLTS SYLDDQDLFV EKLNPDDPEE YLTPDGYKRF
QTRPSIIEIK DAPPITLTLR WTENGPVLPG TFYNLGTITP RGHVMSLSWT LLSPRDTSMS
ASMALMNASN VREAIAATEG FIAPSQNLSL ADRSTIAMKT IGAMPKRDPR HHSKGRLPSQ
GWLPENRWQG RLPYSSNPEF VSPAGGLLGN TNNKIIDRAF PEHVSFAWGD TQRVHRWEKL
MQDREVHTRD SFIEAQLDTV SFTARSLLPL IGADLWYTGE SAPEGTPERQ RQIALALLAE
WNGEMNEYMP EPLIYAAWIR ALQKRLIEDD LGPLADEFKH VEPLFIERVY RDIDGASAWC
DVRQSAPVET CTDMARMALD DALIWIAERY GSQLESLRWG DAHQAAHDHQ VLGEVPLLRY
FVNIRQSTSG GDNTLQRGRT SGEDPNPFLN IHGAGYRGVY DFADPDSSVF ITSTGQSGHF
LSRYYDDQAQ LWRRGEYVPM SLDAELARAA AVGVTHLIPR
//
