ID A0A101E865_9FIRM Unreviewed; 560 AA.
AC A0A101E865;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=XD50_1255 {ECO:0000313|EMBL:KUK10503.1};
OS Clostridia bacterium 41_269.
OC Bacteria; Bacillota; Clostridia.
OX NCBI_TaxID=1635275 {ECO:0000313|EMBL:KUK10503.1, ECO:0000313|Proteomes:UP000053409};
RN [1] {ECO:0000313|Proteomes:UP000053409}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=26787827; DOI=10.1128/mbio.01669-15;
RA Hu P., Tom L., Singh A., Thomas B.C., Baker B.J., Piceno Y.M.,
RA Andersen G.L., Banfield J.F.;
RT "Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and
RT Other Microbial Community Members in Biogeochemical Transformations in Oil
RT Reservoirs.";
RL MBio 7:e01669-15(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUK10503.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LGEZ01000013; KUK10503.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101E865; -.
DR STRING; 1635275.XD50_1255; -.
DR PATRIC; fig|1635275.3.peg.974; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000053409; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
DR PROSITE; PS00722; FTHFS_2; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:KUK10503.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}.
FT BINDING 69..76
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 560 AA; 60680 MW; 856D386533D4BD72 CRC64;
MERPIPSDIE IALKAKKKPI MEIAENLGIK GDDLELFGKY KAKVSLDLMK RLEKTPDGKL
ILVTAITPTP AGEGKTTNSI GLTDALNRLG KKAVVALREP SLGPNFGIKG GAAGGGYSQV
VPMEDINLHF TGDIHAVTYA HNLLAAMIDN HIHQGNELNI DPRKIVFRRV LDLNDRALRF
VVCGLGGPKN GVPRETGFDI SVASEIMAIL CLSLNLEDLK ERMGRIVVGY TYDDKPVTAA
QLEAHGAMAV LMKDAIKPNL VQTLENNPAF IHGGPFANIA HGCNSIIATK MALKLADYVV
TEAGFGADLG AEKFFNLKCR YGGLRPSGVV VVASVRALKF HGGISRECLG EENLSVLKKG
FENLKKHVEN IQKFGLSAVV AINHFPEDSE RELDCLLDMC SQIGVPAAVS EVWEKGSKGG
LELAEKLLDI LENKGEDFRL LYDLELPIKE KIEIIAKEIY GADGVDYSPT ALNQIEKFEH
MGYGKLPICM AKTQYSLSDD PAKIGRPRGF TINVREVRLS AGAGFIVPVT GDIMTMPGLP
KKPAACKMDI DADGTIRGLF
//
