ID A0A101HT06_9FIRM Unreviewed; 645 AA.
AC A0A101HT06;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE Flags: Fragment;
GN ORFNames=XD97_0383 {ECO:0000313|EMBL:KUK82641.1};
OS Pelotomaculum thermopropionicum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfotomaculaceae;
OC Pelotomaculum.
OX NCBI_TaxID=110500 {ECO:0000313|EMBL:KUK82641.1, ECO:0000313|Proteomes:UP000054705};
RN [1] {ECO:0000313|Proteomes:UP000054705}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=26787827; DOI=10.1128/mbio.01669-15;
RA Hu P., Tom L., Singh A., Thomas B.C., Baker B.J., Piceno Y.M.,
RA Andersen G.L., Banfield J.F.;
RT "Genome-Resolved Metagenomic Analysis Reveals Roles for Candidate Phyla and
RT Other Microbial Community Members in Biogeochemical Transformations in Oil
RT Reservoirs.";
RL MBio 7:e01669-15(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUK82641.1}.
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DR EMBL; LGGS01000077; KUK82641.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101HT06; -.
DR Proteomes; UP000054705; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 5..284
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 285..560
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
FT NON_TER 645
FT /evidence="ECO:0000313|EMBL:KUK82641.1"
SQ SEQUENCE 645 AA; 73305 MW; 78CBDAB7F03BB34C CRC64;
MTVLERLNPD QIKAVTYGDG PLLVLAGAGS GKTSVLTRRV AYLLKERRIS PYNILAITFT
NKAAREMRER VEALVPEEVR ALWICTFHAA GLRILRRQTT FYGRDRNFIV YDAGDQLTVV
KDCLKELEID EKKYPPRAVA WAVSQAKNRL LDATGFQRDA DDHFSRTVAE VYRLYQDRLQ
RNNAVDFDDL IFLVVRLFQE YPQVLSYYRD RFRHILVDEY QDTNHAQYVL VNLLAGAHRN
LFAVGDPDQG IYGWRGADIG NIMKFEKDYS EACVVKLEQN YRSTGTILDA ANYIIKNNYN
RKEKRLWTAA DPGTPLVKHY CENEHDEAGF VAGSILRLKE IENRPYRDFA VLYRTHAQSR
VIEETFVRGG VPYTIIGGLK FYERKEIKDI IAYLRLILNH SDALSLSRIA NVPRRGIGNA
SLKKIFAFAG ERGISPVESL ARAVEIPGLT AKVRAGGAAL GTMLIEFSEA AVHSGVTSLT
RTVLEQTGYL QELLAEKTVE ARTRIENLEE FLSVTGDFDR QAEEKGLRSF LENIALVTDL
DQYDGDTDQV ALMTLHSAKG LEFPVVFLTG MEEGVFPHAR SLTEPGELEE ERRLCYVGVT
RAKERVYLTN CWRRTLYGNQ RYNPPSRFLE EIPPHLVING DLVEG
//
