ID   A0A101NDW6_9ACTN        Unreviewed;       400 AA.
AC   A0A101NDW6;
DT   13-APR-2016, integrated into UniProtKB/TrEMBL.
DT   13-APR-2016, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=Serine protease {ECO:0000313|EMBL:KUM91344.1};
GN   ORFNames=AQI88_37520 {ECO:0000313|EMBL:KUM91344.1};
OS   Streptomyces cellostaticus.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=67285 {ECO:0000313|EMBL:KUM91344.1, ECO:0000313|Proteomes:UP000054241};
RN   [1] {ECO:0000313|EMBL:KUM91344.1, ECO:0000313|Proteomes:UP000054241}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40189 {ECO:0000313|EMBL:KUM91344.1,
RC   ECO:0000313|Proteomes:UP000054241};
RA   Ruckert C., Winkler A., Kalinowski J., Kampfer P., Glaeser S.;
RT   "Draft genome sequence of Streptomyces cellostaticus DSM 40189, type strain
RT   for the species Streptomyces cellostaticus.";
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase S8 family.
CC       {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KUM91344.1}.
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DR   EMBL; LMWL01000080; KUM91344.1; -; Genomic_DNA.
DR   RefSeq; WP_067008870.1; NZ_KQ948049.1.
DR   AlphaFoldDB; A0A101NDW6; -.
DR   STRING; 67285.AQI88_37520; -.
DR   OrthoDB; 9798386at2; -.
DR   Proteomes; UP000054241; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR   InterPro; IPR000209; Peptidase_S8/S53_dom.
DR   InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR   InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR   PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR   PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR   Pfam; PF00082; Peptidase_S8; 1.
DR   PRINTS; PR00723; SUBTILISIN.
DR   SUPFAM; SSF52743; Subtilisin-like; 1.
DR   PROSITE; PS51892; SUBTILASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Membrane {ECO:0000256|SAM:Phobius};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000054241};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW   ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..400
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5038565643"
FT   TRANSMEM        374..394
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          45..297
FT                   /note="Peptidase S8/S53"
FT                   /evidence="ECO:0000259|Pfam:PF00082"
FT   REGION          309..369
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        328..369
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        54
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        88
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT   ACT_SITE        248
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
SQ   SEQUENCE   400 AA;  41006 MW;  1A70E20A10C8B5E6 CRC64;
     MCSALGTLLI ASAGLAPGAA AEDIQSKQWY LNSMGADEIW KYSTGKGVKV AVIDSGVNPA
     TSTLKGQVLV NQVPKSVAYK AAEDNDGHGT GMAEIIAGTG AGGGVKGLAP GAKIIPYHVQ
     QTTDRKDDDG KFASLADVIR AAADTDVKII NMSFGGPYPD STEEDAIKYA ASKGKLMFAS
     IGNDGEKGKA EYPAVYPYVV GVSAIDKSGT VAKFSTKSNY VDFAAPGVSV PGWCDGKFTE
     YCDTDGTSPA TALASASAAL VWSVHPDWTA NQVLRTLLDT AGRSWPKDKP STYLGYGAIR
     PRRVLINKNI DPGPANRDPL YAENGTDPNE DVTATPSLSV APSSGATAKD SGSTSGATSA
     AAKDSGSGSN TTTWVIIGAV AAVLVIGGAG FGVLRARRNA
//