ID A0A101T1I6_9ACTN Unreviewed; 464 AA.
AC A0A101T1I6;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE SubName: Full=Arabinase {ECO:0000313|EMBL:KUN84072.1};
GN ORFNames=AQJ64_16565 {ECO:0000313|EMBL:KUN84072.1};
OS Streptomyces griseoruber.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1943 {ECO:0000313|EMBL:KUN84072.1, ECO:0000313|Proteomes:UP000052982};
RN [1] {ECO:0000313|EMBL:KUN84072.1, ECO:0000313|Proteomes:UP000052982}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40281 {ECO:0000313|EMBL:KUN84072.1,
RC ECO:0000313|Proteomes:UP000052982};
RA Ruckert C., Winkler A., Kalinowski J., Kampfer P., Glaeser S.;
RT "Draft genome sequence of Streptomyces griseoruber DSM 40281, type strain
RT for the species Streptomyces griseoruber.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUN84072.1}.
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DR EMBL; LMWW01000019; KUN84072.1; -; Genomic_DNA.
DR RefSeq; WP_055635616.1; NZ_LIQS01000294.1.
DR AlphaFoldDB; A0A101T1I6; -.
DR STRING; 1943.AQJ64_16565; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000052982; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..31
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 32..464
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5007106792"
FT DOMAIN 327..464
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 40
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 211
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 161
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 464 AA; 48691 MW; 4DE7C6498F313D14 CRC64;
MRARRGRSAP AAALIAFLTV LGLVTASPAQ ALSGDTRMHD PSLIKVGSCY YGFSTGFEND
PLNPSGSITI RKTCAATAAS GWTKVGNVWE STPSWITAKL GSTPPNIWAP DVKYFNGKYH
LYYAGSLWGS AYAVMGVATA TDIEGPWTDQ GMVTDVNYPI DPNVDWGPDG RLYITWGSFT
GPGTYMHVLD QATGKLSTTD NNLWHLAVGI ENPTIILNGG FYYLFGSKGL CCSGTNSNYY
TVVGRSTSIT GPYLDQSGTN MLSNGGTTVL TGARPKVAGG GADAYDDGAL KFLAYHYYDG
DNSGRETLDI RQVTFANGWP VLAGPLGAAN NHLLNRNADT CADVWFASTA DGASVNSGNC
NSGTNQQWVS TAVGSPYELV NVNSGKCLQI AGASTADGAV AVQSTCTGAS HQLWKKTPVV
GGYVTFTNVN SGKCLEVAGA STTNGAALDQ STCTTGANHQ WMIV
//
