ID A0A101UG67_9ACTN Unreviewed; 614 AA.
AC A0A101UG67;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:KUO10110.1};
GN ORFNames=AQJ58_21935 {ECO:0000313|EMBL:KUO10110.1};
OS Streptomyces sp. DSM 15324.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1739111 {ECO:0000313|EMBL:KUO10110.1, ECO:0000313|Proteomes:UP000053431};
RN [1] {ECO:0000313|EMBL:KUO10110.1, ECO:0000313|Proteomes:UP000053431}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 15324 {ECO:0000313|EMBL:KUO10110.1,
RC ECO:0000313|Proteomes:UP000053431};
RA Ruckert C., Winkler A., Kalinowski J., Wink J., Kampfer P., Glaeser S.;
RT "Draft genome sequence of Streptomyces sp. DSM 15324, isolated from soil.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUO10110.1}.
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DR EMBL; LMXA01000019; KUO10110.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A101UG67; -.
DR Proteomes; UP000053431; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; NF033712; B12_rSAM_KedN5; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF18; RADICAL SAM SUPERFAMILY PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDF00436; pactamycin_C-methyltransferase; 1.
DR SFLD; SFLDF00317; thioacetal_methlytransferase; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000053431}.
FT DOMAIN 1..126
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 175..404
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 614 AA; 70733 MW; BC305E23E8CAA1CC CRC64;
MPLAIGYLKA YAEADERIRR HMDISLRNYP GDAGLNAMAR DLVRDGCPDI LCFSVLGWNF
RAFGALAETF KQLNPNGWVV FGGNHVAHQG ERVFRTFPQV DVVVNGEGEL VFRDLMNACL
DGAGRDGLHG IAGISFREAG GDLVTTPERE RIKDLEILPS PILTGAIPLV DRQGRFLYDY
ALMETNRGCP YKCAFCYWGG ATGQKMRAFS RERLRAELEV LGRRGAEILM LADSNFGLLR
QDEEFLDDLL RVRRKYGFPR RLETSWAKNK SPGFYRIMEK MKQSGMHSAF ILALQTMDDS
VLDLMRRRNM KLNDWESLVA WLTDHGITPY LELIWGAPGE TVASFLDGYD RAARHTPFIA
VHPLMLLPNT EYHDKKEQHG MVTVRGEQDD FDYVLAHRTM TLADNERMLR FICWNRVLAR
SLWLHNIWVA LRELADVPQS RVILSFSDWI DKSEDPEARE LHALARPTSS ASENVDPNVW
RLLTKRLLRL WWEEALRPGL PEELLPLLDE VFRYDLMCQP VRPLPDGSGP EEDLPVVTRH
GERWYLRSDT EFTHPVPRLI AALRRGESVS TRPERHTADF YYRTEFGGDL QHYFRMDRFR
GLTEQQLEQQ FSQA
//
