ID   A0A101VVF0_9FIRM        Unreviewed;      1407 AA.
AC   A0A101VVF0;
DT   13-APR-2016, integrated into UniProtKB/TrEMBL.
DT   13-APR-2016, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   SubName: Full=2-hydroxyglutaryl-CoA dehydratase {ECO:0000313|EMBL:KUO61345.1};
GN   ORFNames=APF84_11735 {ECO:0000313|EMBL:KUO61345.1};
OS   Gracilibacter sp. BRH_c7a.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Gracilibacteraceae;
OC   Gracilibacter.
OX   NCBI_TaxID=1734398 {ECO:0000313|EMBL:KUO61345.1, ECO:0000313|Proteomes:UP000053404};
RN   [1] {ECO:0000313|EMBL:KUO61345.1, ECO:0000313|Proteomes:UP000053404}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRH_c7a {ECO:0000313|EMBL:KUO61345.1};
RA   Bagnoud A., Chourey K., Hettich R.L., De Bruijn I., Andersson A.F.,
RA   Leupin O.X., Schwyn B., Bernier-Latmani R.;
RT   "Microbial metabolic network in the subsurface.";
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KUO61345.1}.
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DR   EMBL; LOEZ01000067; KUO61345.1; -; Genomic_DNA.
DR   STRING; 1734398.APF84_11735; -.
DR   Proteomes; UP000053404; Unassembled WGS sequence.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR   Gene3D; 3.30.420.40; -; 4.
DR   InterPro; IPR002731; ATPase_BadF.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR018709; CoA_activase_DUF2229.
DR   InterPro; IPR008275; CoA_E_activase_dom.
DR   NCBIfam; TIGR00241; CoA_E_activ; 2.
DR   PANTHER; PTHR32329:SF4; ACTIVATOR OF 2-HYDROXYACYL-COA DEHYDRATASE-RELATED; 1.
DR   PANTHER; PTHR32329; BIFUNCTIONAL PROTEIN [INCLUDES 2-HYDROXYACYL-COA DEHYDRATASE (N-TER) AND ITS ACTIVATOR DOMAIN (C_TERM)-RELATED; 1.
DR   Pfam; PF01869; BcrAD_BadFG; 2.
DR   Pfam; PF09989; DUF2229; 1.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 2.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023014};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00023014}.
FT   DOMAIN          11..259
FT                   /note="ATPase BadF/BadG/BcrA/BcrD type"
FT                   /evidence="ECO:0000259|Pfam:PF01869"
FT   DOMAIN          325..579
FT                   /note="ATPase BadF/BadG/BcrA/BcrD type"
FT                   /evidence="ECO:0000259|Pfam:PF01869"
FT   DOMAIN          673..892
FT                   /note="DUF2229"
FT                   /evidence="ECO:0000259|Pfam:PF09989"
SQ   SEQUENCE   1407 AA;  157589 MW;  6A8843D13AF10C5F CRC64;
     MLEEKPLYFF GIDVGSTTVK IAVLDEKDNL IFSRYKRHYS DIWKTLQELI QEVHQELPNP
     RIKITVTGSG GMSISEQLDI TFVQEVIAGS KAIDRFLPGT DVVIELGGED AKITFYNNGV
     DQRMNGICAG GTGAFIDQMA ILLKTDAEGL NRLAQDHRVI YPIAARCGVF AKTDIQPLLN
     EGARKEDIAV SIFQAVVNQT IAGLACGRRI HGKVVLLGGP VYFLPQLRLR FKETLNLEDE
     NIIVPDNSQI YVAVGAALAS KKEEEVTLDN LKSKFNLDRK SLLYETGKLE PLFADNIEYK
     RFVQRHDRNK VKRKELNSFQ GKAFLGMDVG STTTKIALID GRGNLLYYDY GNNGGNPLES
     AVNMLKKLYK SLTPELEIAH CAVTGYGENL VKNALCFDIG EVETIAHYTA AEYFLPGVNF
     ILDIGGQDMK SLKIQDGLIE NIMLNEACSS GCGSFIETFA QSLEMDIQDF ARQGLLAQAP
     ADLGSRCTVF MNSRVKQAQK EGVSVGEISA GLSYSVIKNA LYKVIKIKGS EDLGEKIIVQ
     GGTFLNDSIV RSLELITGRE VVRPDIAGIM GAFGAALLAS RAWREGVASS LIGVDKIQDF
     TVESRIIRCN KCTNNCLLTV NTFTDGRKLI TGNRCEKGAG LEKSEEIPNL YEYKTKRLFS
     YTPLQRDKAI RGTIGIPRVL NMYENYPFWF TFFTRLGFRV EISPESNKKL FETGMDTISS
     DTACYPAKIV HGHIVSLVNQ GVKLIFYPCV PKERQEMKKA DNCFNCPIVI GYAEVIRANM
     DILKQNNVRF LNPFLPYHNK KRLTKRLFEE LREFGVSLEE TAKAVEAAWE EDLKSKRDIR
     KKGEEILAWL QENGKRGIVL SGRPYHLDLE VNHGIPNIIT SLGMAVLTED SVAHLGKVKR
     PLRVLDQWMY HSRLYEATDF VSQQDNLELV QLVSFGCGPD SIAAEQAEEI LHRTGKIYTM
     IKIDEVSNLG AVRIRLRSLK AAVEARQGRQ NKNKSDVYSI PQRPVFTKEM RKSHTILAPQ
     MAPIHFEFTQ EVARSEGYNF VVLAEVDNKD IEEGLKYVNN DSCYPSIIII GQVLRALKTG
     KYDLDKTAII MSQTGGPCRA SNYLSLLKKA LHSNGFGHIP IISLNVAGLE KNPGFKITMP
     MFTKAVMGMV YGDILMNVVH RVRPYELIPG TTNKLLKKWI EICKVNVRTG ERRVFRENLK
     SIVQDFDNIK IKKNERRPRV GLVGEILVKF HPAANNNMVD FIEKSGAEMV VPGLSEFFLY
     CASSRTYEQK YLAGSNLKKI LGNFFIKYVE SYREDMRNAL RESSRFTVPH TIAEMAESIN
     AILSLGNQTG EGWLLTAEMV ALIEEGAHNI ICMQPFACLP NHITGKGMIK TLKELYPQTN
     IVAIDYDPGA SEVNQINRIK LMLEKAR
//