ID A0A103XWW9_CYNCS Unreviewed; 526 AA.
AC A0A103XWW9;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=3-ketoacyl-CoA synthase {ECO:0000256|PIRNR:PIRNR036417};
DE EC=2.3.1.- {ECO:0000256|PIRNR:PIRNR036417};
GN ORFNames=Ccrd_023373 {ECO:0000313|EMBL:KVH98408.1};
OS Cynara cardunculus var. scolymus (Globe artichoke) (Cynara scolymus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Carduoideae; Cardueae;
OC Carduinae; Cynara.
OX NCBI_TaxID=59895 {ECO:0000313|EMBL:KVH98408.1, ECO:0000313|Proteomes:UP000243975};
RN [1] {ECO:0000313|EMBL:KVH98408.1, ECO:0000313|Proteomes:UP000243975}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2C {ECO:0000313|EMBL:KVH98408.1};
RX PubMed=26786968; DOI=10.1038/srep19427;
RA Scaglione D., Reyes-Chin-Wo S., Acquadro A., Froenicke L., Portis E.,
RA Beitel C., Tirone M., Mauro R., Lo Monaco A., Mauromicale G., Faccioli P.,
RA Cattivelli L., Rieseberg L., Michelmore R., Lanteri S.;
RT "The genome sequence of the outbreeding globe artichoke constructed de novo
RT incorporating a phase-aware low-pass sequencing strategy of F1 progeny.";
RL Sci. Rep. 6:19427-19427(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a very-long-chain acyl-CoA + H(+) + malonyl-CoA = a very-long-
CC chain 3-oxoacyl-CoA + CO2 + CoA; Xref=Rhea:RHEA:32727,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57384, ChEBI:CHEBI:90725, ChEBI:CHEBI:90736;
CC EC=2.3.1.199; Evidence={ECO:0000256|ARBA:ARBA00001906};
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194, ECO:0000256|PIRNR:PIRNR036417}.
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC synthases family. {ECO:0000256|ARBA:ARBA00005531,
CC ECO:0000256|PIRNR:PIRNR036417}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KVH98408.1}.
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DR EMBL; LEKV01003799; KVH98408.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A103XWW9; -.
DR STRING; 59895.A0A103XWW9; -.
DR EnsemblPlants; KVH98408; KVH98408; Ccrd_023373.
DR Gramene; KVH98408; KVH98408; Ccrd_023373.
DR OMA; HHSKLCG; -.
DR OrthoDB; 930987at2759; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000243975; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0009922; F:fatty acid elongase activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00831; CHS_like; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR InterPro; IPR012392; 3-ktacl-CoA_syn.
DR InterPro; IPR013747; ACP_syn_III_C.
DR InterPro; IPR013601; FAE1_typ3_polyketide_synth.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR31561; 3-KETOACYL-COA SYNTHASE; 1.
DR PANTHER; PTHR31561:SF99; 3-KETOACYL-COA SYNTHASE 4; 1.
DR Pfam; PF08541; ACP_syn_III_C; 1.
DR Pfam; PF08392; FAE1_CUT1_RppA; 1.
DR PIRSF; PIRSF036417; 3-ktacl-CoA_syn; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|PIRNR:PIRNR036417};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000243975};
KW Transferase {ECO:0000256|PIRNR:PIRNR036417};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 55..76
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 97..116
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 114..403
FT /note="FAE"
FT /evidence="ECO:0000259|Pfam:PF08392"
FT DOMAIN 420..500
FT /note="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF08541"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 258
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 337
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 421
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 425
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 454
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
FT ACT_SITE 458
FT /evidence="ECO:0000256|PIRSR:PIRSR036417-1"
SQ SEQUENCE 526 AA; 58243 MW; 1028FE1F152CCCFC CRC64;
MDGGANLNTN GGGGGGGGGG GGGPTVGVQI NQRHSHRRLP DFLQSVNLKY VKLGYHYLIS
HLLTLCLVPV MIVILIEASQ MNPSDIRQLW VHLQYNLVSI IICSAVLVFG STVYIMTRPK
PVYLVDYACY RPPDHLKAPY ERFMKHSRLT GDFDESSLEF QRKILERSGL GEETYVPEAM
HFVPPRPSMA AAREEAEQVM YGALDNLFAA TGIKPKDIGI LVVNCSLFNP TPSLSSMIVN
KYKLRGNIRS FNLGGMGCSA GVIAVDLAKD MLQVHRNTYA VVVSTENITQ NWYFGNKKSM
LIPNCLFRVG GSAVLLSNKS VDRSRAKYKL VHVVRTHRGA DDKAFRCVYQ EQDPAGKTGV
SLSKDLMAIA GGALKTNITT LGPLVLPISE QLLFFCTLIA KKLFNDDVKP YIPDFKLAFD
HFCIHAGGRA VIDELEKNLQ LLPTHVEASR MTLHRFGNTS SSSIWYELAY TESKGRMRKG
HRVWQIAFGS GFKCNSAVWK ALRNVKPPVN GPWEDCIDKY PVELVS
//
