ID A0A108THF5_9BACE Unreviewed; 189 AA.
AC A0A108THF5;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Crossover junction endodeoxyribonuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
DE EC=3.1.21.10 {ECO:0000256|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction nuclease RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
DE AltName: Full=Holliday junction resolvase RuvC {ECO:0000256|HAMAP-Rule:MF_00034};
GN Name=ruvC {ECO:0000256|HAMAP-Rule:MF_00034,
GN ECO:0000313|EMBL:KAA5409800.1};
GN ORFNames=DWW88_04220 {ECO:0000313|EMBL:RGU29743.1}, DWX97_26095
GN {ECO:0000313|EMBL:RGS30566.1}, F2Y53_01945
GN {ECO:0000313|EMBL:KAA5466743.1}, F2Y70_06305
GN {ECO:0000313|EMBL:KAA5427115.1}, F2Y81_03895
GN {ECO:0000313|EMBL:KAA5422600.1}, F2Y86_08830
GN {ECO:0000313|EMBL:KAA5409800.1}, F2Y87_24810
GN {ECO:0000313|EMBL:KAA5413936.1};
OS Bacteroides cellulosilyticus.
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
OC Bacteroides.
OX NCBI_TaxID=246787 {ECO:0000313|EMBL:RGU29743.1, ECO:0000313|Proteomes:UP000285677};
RN [1] {ECO:0000313|Proteomes:UP000283341, ECO:0000313|Proteomes:UP000285677}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AF17-25 {ECO:0000313|EMBL:RGU29743.1,
RC ECO:0000313|Proteomes:UP000285677}, and AF22-3AC
RC {ECO:0000313|EMBL:RGS30566.1, ECO:0000313|Proteomes:UP000283341};
RA Zou Y., Xue W., Luo G.;
RT "A genome reference for cultivated species of the human gut microbiota.";
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000325055, ECO:0000313|Proteomes:UP000425249}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIOML-A2 {ECO:0000313|EMBL:KAA5466743.1,
RC ECO:0000313|Proteomes:UP000425249}, BIOML-A3
RC {ECO:0000313|EMBL:KAA5427115.1, ECO:0000313|Proteomes:UP000475645},
RC BIOML-A6 {ECO:0000313|EMBL:KAA5422600.1,
RC ECO:0000313|Proteomes:UP000448877}, BIOML-A7
RC {ECO:0000313|EMBL:KAA5409800.1, ECO:0000313|Proteomes:UP000325055},
RC and BIOML-A8 {ECO:0000313|EMBL:KAA5413936.1,
RC ECO:0000313|Proteomes:UP000482653};
RX PubMed=31477907; DOI=.1038/s41591-019-0559-3;
RA Poyet M., Groussin M., Gibbons S.M., Avila-Pacheco J., Jiang X.,
RA Kearney S.M., Perrotta A.R., Berdy B., Zhao S., Lieberman T.D.,
RA Swanson P.K., Smith M., Roesemann S., Alexander J.E., Rich S.A., Livny J.,
RA Vlamakis H., Clish C., Bullock K., Deik A., Scott J., Pierce K.A.,
RA Xavier R.J., Alm E.J.;
RT "A library of human gut bacterial isolates paired with longitudinal
RT multiomics data enables mechanistic microbiome research.";
RL Nat. Med. 25:1442-1452(2019).
CC -!- FUNCTION: The RuvA-RuvB-RuvC complex processes Holliday junction (HJ)
CC DNA during genetic recombination and DNA repair. Endonuclease that
CC resolves HJ intermediates. Cleaves cruciform DNA by making single-
CC stranded nicks across the HJ at symmetrical positions within the
CC homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group;
CC requires a central core of homology in the junction. The consensus
CC cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side
CC of the TT dinucleotide at the point of strand exchange. HJ branch
CC migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds
CC its consensus sequence, where it cleaves and resolves the cruciform
CC DNA. {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage at a junction such as a reciprocal
CC single-stranded crossover between two homologous DNA duplexes
CC (Holliday junction).; EC=3.1.21.10; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00034};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00034};
CC Note=Binds 2 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00034};
CC -!- SUBUNIT: Homodimer which binds Holliday junction (HJ) DNA. The HJ
CC becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it
CC has a different conformation from HJ DNA in complex with RuvA. In the
CC full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms
CC which resolves the HJ. {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- SIMILARITY: Belongs to the RuvC family. {ECO:0000256|ARBA:ARBA00009518,
CC ECO:0000256|HAMAP-Rule:MF_00034}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RGU29743.1}.
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DR EMBL; VVYW01000006; KAA5409800.1; -; Genomic_DNA.
DR EMBL; VVYX01000043; KAA5413936.1; -; Genomic_DNA.
DR EMBL; VVYV01000004; KAA5422600.1; -; Genomic_DNA.
DR EMBL; VVYT01000012; KAA5427115.1; -; Genomic_DNA.
DR EMBL; VVYS01000022; KAA5466743.1; -; Genomic_DNA.
DR EMBL; QRVJ01000047; RGS30566.1; -; Genomic_DNA.
DR EMBL; QRXS01000002; RGU29743.1; -; Genomic_DNA.
DR RefSeq; WP_007214744.1; NZ_VVYX01000043.1.
DR AlphaFoldDB; A0A108THF5; -.
DR STRING; 246787.BcellWH2_01267; -.
DR eggNOG; COG0817; Bacteria.
DR Proteomes; UP000283341; Unassembled WGS sequence.
DR Proteomes; UP000285677; Unassembled WGS sequence.
DR Proteomes; UP000325055; Unassembled WGS sequence.
DR Proteomes; UP000425249; Unassembled WGS sequence.
DR Proteomes; UP000448877; Unassembled WGS sequence.
DR Proteomes; UP000475645; Unassembled WGS sequence.
DR Proteomes; UP000482653; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0048476; C:Holliday junction resolvase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0008821; F:crossover junction DNA endonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd16962; RuvC; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_00034; RuvC; 1.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR020563; X-over_junc_endoDNase_Mg_BS.
DR InterPro; IPR002176; X-over_junc_endoDNase_RuvC.
DR NCBIfam; TIGR00228; ruvC; 1.
DR PANTHER; PTHR30194; CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVC; 1.
DR PANTHER; PTHR30194:SF3; CROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVC; 1.
DR Pfam; PF02075; RuvC; 1.
DR PRINTS; PR00696; RSOLVASERUVC.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS01321; RUVC; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00034};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000256|HAMAP-
KW Rule:MF_00034};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00034, ECO:0000313|EMBL:RGU29743.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00034};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00034};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00034}.
FT ACT_SITE 14
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT ACT_SITE 74
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT ACT_SITE 149
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 14
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 74
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
FT BINDING 149
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00034"
SQ SEQUENCE 189 AA; 21113 MW; F75949FE039597C8 CRC64;
MIQPVKEKII LGIDPGTTIM GYGVLRVQGT KPEMIAMGII DLRKMGDHYL KLRHIHERVL
SIIESYLPDE LAIEAPFFGK NVQSMLKLGR AQGVAMAAAL SRDIPITEYA PLKIKMAITG
NGQASKEQVA DMLQRMLHFA KEDMPTFMDA TDGLAAAYCH FLQMGRPTVE KGYHGWKDFI
AKNPDKVKR
//
