ID A0A109MUM2_9BACI Unreviewed; 462 AA.
AC A0A109MUM2;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=RNA methyltransferase {ECO:0000313|EMBL:KWW15532.1};
GN ORFNames=AS888_08350 {ECO:0000313|EMBL:KWW15532.1};
OS Peribacillus simplex.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Peribacillus.
OX NCBI_TaxID=1478 {ECO:0000313|EMBL:KWW15532.1, ECO:0000313|Proteomes:UP000064189};
RN [1] {ECO:0000313|EMBL:KWW15532.1, ECO:0000313|Proteomes:UP000064189}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VanAntwerpen02 {ECO:0000313|EMBL:KWW15532.1,
RC ECO:0000313|Proteomes:UP000064189};
RA Couger M.B.;
RT "Genome Sequence of Bacillus simplex strain VanAntwerpen2.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KWW15532.1}.
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DR EMBL; LNNH01000039; KWW15532.1; -; Genomic_DNA.
DR RefSeq; WP_061143620.1; NZ_LNNH01000039.1.
DR AlphaFoldDB; A0A109MUM2; -.
DR Proteomes; UP000064189; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061:SF45; -; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 8..66
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 417
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 417
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 292
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 321
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 342
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 390
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 462 AA; 51881 MW; BF37CC7B090A2ABD CRC64;
MTNIQDTQLE VNQTLPLTIK RLGINGEGVG YFKKKVVFVP GALPGEEIVA SVTKVQPNFT
EAKIKTIRKE SPHRITAPCP VYAECGGCQL QHLSYDQQLV EKRDIVIQAM ERHTKLPVFS
LNIKETIGME NPWNYRNKSQ YQVGQKDGKL IAGLYGLNSH TLIDIPNCLV QHKATNKVTR
TVKKILKNLN ISIYNERKRK GVIRTVITRV GFETGEVQVV LVTGAEEIPQ KDQLLKQIRD
QLPEVKSVVQ NINNRNTSLI FGEKTIHLAG GKVINETLGD LSYELSARTF FQLNPVQTVR
MYDEVKKAAA LTGTEKVVDA YCGVGTIGLW LSEEAKEVRG MDVIKESIED AKKNAKKHKR
SNMHYETGKA ENILPRWIKE GWKPDMLVVD PPRTGCDQAL LQTILKAKPK KIVYVSCNPS
TLAKDLQELS TIYEVQSIQP VDMFPQTSHV ECVSQLILKE GN
//
