ID A0A117MPQ1_9ACTN Unreviewed; 1184 AA.
AC A0A117MPQ1;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ADL12_28035 {ECO:0000313|EMBL:KUL28959.1};
OS Streptomyces regalis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=68262 {ECO:0000313|EMBL:KUL28959.1, ECO:0000313|Proteomes:UP000053923};
RN [1] {ECO:0000313|Proteomes:UP000053923}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL 3151 {ECO:0000313|Proteomes:UP000053923};
RA Ju K.-S., Doroghazi J.R., Metcalf W.W.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUL28959.1}.
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DR EMBL; LLZG01000334; KUL28959.1; -; Genomic_DNA.
DR RefSeq; WP_062706594.1; NZ_LLZG01000334.1.
DR AlphaFoldDB; A0A117MPQ1; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000053923; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000053923}.
FT DOMAIN 649..810
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 835..985
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1184 AA; 129245 MW; DB2BB49FBB2D0BBF CRC64;
MSLHGLLDAV VKDPALAEAI TAAADGNRMH VDLVGPPAAR PFTIAALARE TDRPVLAVTA
TGREAEDLAA ALRSLLPSEG VVEYPSWETL PHERLSPRSD TVGRRLAVLR RLAHPRPDDP
ETGPLSVVVA PVRSVLQPQV KGLGDLEPVA LRTGQTADLN AIVEALAAAA YARVELVEKR
GEFAVRGGIL DVFPPTEEHP LRIEFWGDDV EEIRYFKVAD QRSLEVAEHG LWAPPCRELL
LTDDVRARAR ALAEQHPELG ELLGKIAEGI AVEGMESLAP VLVDDMELLL DVLPKGAMAV
VCDPERVRTR AADLVATSQE FLHASWAATA GGGEAPIDVG AASLWSIADV RDRARELDMM
WWSVSPFAAD DTFTFAADAA GDADTLKLGM HAPESYRGDT AKALADTKGW LADGWRTVFV
TEAHGPAART VEVLGGEGVA ARLEADLGEI APSVVHVACG SIDYGFVDPV LKLAVLTETD
LSGQKAAGKD GARMPARRRK TIDPLTLEAG DYIVHEQHGV GRYIEMVQRT VQGATREYLV
VEYAPAKRGQ PGDRLYIPTD QLEQITKYVG GEAPTLHRLG GADWTKTKAR AKKAVKEIAA
DLIKLYSARM AAPGHTFGPD TPWQRELEDA FPYAETPDQL TTIAEVKEDM EKSVPMDRLI
CGDVGYGKTE IAVRAAFKAV QDGKQVAVLV PTTLLVQQHF GTFSERYSQF PVNTRALSRF
QTDTEAKATL EGLKDGSVDI VIGTHRLFSS ETKFKDLGLV IVDEEQRFGV EHKEQLKKLR
ANVDVLTMSA TPIPRTLEMA VTGIREMSTI TTPPEERHPV LTFVGPYEEK QIGAAIRREL
LREGQVFYIH NRVESIDRAA ARLREIVPEA RIATAHGQMS EQSLEQVVVD FWEKKFDVLV
STTIVESGID ISNANTLIVE RGDNFGLSQL HQLRGRVGRG RERGYAYFLY PPEKPLTETA
HERLATIAQH TEMGAGMYVA MKDLEIRGAG NLLGGEQSGH IAGVGFDLYV RMVGEAVADY
RASLEGGVEE EPPLEVKIEL PVDAHVPHDY APGERLRLQA YRSIASANTE EDIKAVREEL
VDRYGKLPEP VENLLLVAGL RMLARACGVG EIVLQGNNIR FAPVELRESQ ELRVKRLYPG
TVIKPAVHQL LVPRPKTAKV GGKPLVGREL LGWVGEFLAS ILGS
//
