ID A0A117MPR8_9ACTN Unreviewed; 1194 AA.
AC A0A117MPR8;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=ADL15_29745 {ECO:0000313|EMBL:KUL29067.1};
OS Actinoplanes awajinensis subsp. mycoplanecinus.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Actinoplanes.
OX NCBI_TaxID=135947 {ECO:0000313|EMBL:KUL29067.1, ECO:0000313|Proteomes:UP000053244};
RN [1] {ECO:0000313|EMBL:KUL29067.1, ECO:0000313|Proteomes:UP000053244}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-16712 {ECO:0000313|EMBL:KUL29067.1,
RC ECO:0000313|Proteomes:UP000053244};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUL29067.1}.
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DR EMBL; LLZH01000282; KUL29067.1; -; Genomic_DNA.
DR RefSeq; WP_067698104.1; NZ_LLZH01000282.1.
DR AlphaFoldDB; A0A117MPR8; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000053244; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000053244}.
FT DOMAIN 22..346
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 347..670
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 887..941
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 43..50
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1194 AA; 128125 MW; FED2B78B1EEF725F CRC64;
MRNARVGPRY TPLELARLLH LPPPTDEQAA IIAHPVKPLL VVAGAGSGKT ETMASRVVWL
VANGYAHPGE ILGLTFTRKA AGELAHRVRA RLSQLVRSLG GRDEALAGEA TISTYHSYAA
RVVTEHGLRG GFEPSARLLT EAARWQIVDS LVRAYTGEMT GLNRAPGTVT DDVLALSAEL
AEHLLTPDDL AAWTGRFFAE LQSFPGRMYK DVSESLRRQQ NRLTLLPLVR LYEQRKLGIE
AMDFGDQMAR AALVARDHPE VGRVERGRFR VVLLDEYQDT SHAQVVMLNN LFGGGHPVTA
VGDPCQSIYG WRGASAGTLE RFPDEFPDAE GHPAWVRSLT RSWRNRPEIL KVANVVSAPL
RAQVGVLKPA ERVAESVGGR TVSCALLPSY AEEASWIADS MLTAWRLVAG MPKALPEEIP
LERRPTSAVL VRVRSQIPVI EEALRARGLP VEVVGLGGLL DTPEVRDVVC TLRVLADPTD
GASLLRLLTG ARWRIGPRDL VALHRRARTI AGARAPKPED PESEAPAEVT ADRLDDATLI
EAMADLGAPA QYAPDGYARL AAFSRELGAL RQRLDQPLPD LLADIERTIG LDVEVAVRGW
AAGDAGLARA HLDTLGEAAT RFGSENESGT IAGFLAFLTA AEEEERGLEP GQVDVVEGAV
QILTAHAAKG LEWDVVSAAG LCRGVWPGLV RGSDHYLGGI GVLPFPLRGD ASGLPVLDLE
DATDQKDVAG ALSDFARAWR EHDEREERRL AYVAVTRPRR LLLCSGYWWG DGVKRPRGPS
VFLDEIRATC EAGAGVVAVW TPEPAPGETN PSEEVVATAE WPSDPLGLRR PAMAAAADLI
RRMVADPDAS AAEAFADLAA AHPEVAARAG LAADLVGLAG PGWSVGVAAD PGDTPEDQPG
PHAGALAADA SSDRPFPVRP EPSETAPAAT GPADPVHPGA APALDRASLL AAASAVDRVA
AEVDPDIARW RREARLLLAE RAERAHRDGP IEVALPPHLS VSQLVVLRRD PQALARSLRR
PLPQRPAPHA RRGTAFHVWL EQRFGAGRLI DIDELPGAAD EHAAGDEELL ALQEAFLTGE
WADRTPLDVE VPFATTVAGV VVRGRMDAVF ADPDGRFDVI DWKTGRRPTG AEAEAAAVQL
SAYRIAWATL AGVPITRVRA GFHYVREQVT VRPADLMDVS ALAALITEIP EESL
//
