ID A0A117PAR4_9ACTN Unreviewed; 499 AA.
AC A0A117PAR4;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE SubName: Full=Hydrolase {ECO:0000313|EMBL:KUM76123.1};
GN ORFNames=AQI70_16240 {ECO:0000313|EMBL:KUM76123.1};
OS Streptomyces curacoi.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=146536 {ECO:0000313|EMBL:KUM76123.1, ECO:0000313|Proteomes:UP000054024};
RN [1] {ECO:0000313|EMBL:KUM76123.1, ECO:0000313|Proteomes:UP000054024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 40107 {ECO:0000313|EMBL:KUM76123.1,
RC ECO:0000313|Proteomes:UP000054024};
RA Ruckert C., Winkler A., Kalinowski J., Kampfer P., Glaeser S.;
RT "Draft genome sequence of Streptomyces curacoi DSM 40107, type strain for
RT the species Streptomyces curacoi.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUM76123.1}.
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DR EMBL; LMWJ01000010; KUM76123.1; -; Genomic_DNA.
DR RefSeq; WP_062149753.1; NZ_KQ947987.1.
DR AlphaFoldDB; A0A117PAR4; -.
DR STRING; 146536.AQI70_16240; -.
DR OrthoDB; 177947at2; -.
DR Proteomes; UP000054024; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18820; GH43_LbAraf43-like; 1.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 3.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR InterPro; IPR006311; TAT_signal.
DR PANTHER; PTHR43817; GLYCOSYL HYDROLASE; 1.
DR PANTHER; PTHR43817:SF1; HYDROLASE, FAMILY 43, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G01660)-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:KUM76123.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000054024};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..33
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 34..499
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5007152949"
FT DOMAIN 358..496
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 50
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 218
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 166
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 499 AA; 53683 MW; 4606F3162FD4EC4A CRC64;
MPRTPHTKRR RSLLLCALAA LLALVATTQP ASAADGRPYT NPLKSFKGAD PWLQYHDGNY
YLITTTFTGI LGIRKAPTLA GLATAPNVQV WSDTTSTRNT NFWAPEMHLF NGHWYVYYSA
GQSGVACCDS QRTHVLESAG TDPMGPYTYK GSLTGSNLTP GGWLIDASVL QANNKLYLVG
SGFINGSTQS LVIAPMSNPY TLTGNTFTII SSPTLDWERS GSPVNEGPEP LYRGGRTFLT
YSASSCQTAD YKLGQLELTG ADPLNPASWT KKQTPVFQRS DANGVYGPGH NGFFTSPDGT
ENWIVYHANS SASGGCGNGR TTRAQEFTWN ADGTPNFGTP VALGTTLPGP SGETAATPTA
YTLVNRNSGK CLDVNGGNTA DGTNIFQWTC NGGTNQKWKV EDLGDDTNRL VNVATGKVMD
TAECSAADGA DIRQWSWLNN KCQHYRLVLT ASGDYVRIVN ESTGKVADVA DCGTANGTDV
RQWTWLNNNC QQWKLVPTT
//
