ID   A0A124GUV9_9ACTN        Unreviewed;       293 AA.
AC   A0A124GUV9;
DT   13-APR-2016, integrated into UniProtKB/TrEMBL.
DT   13-APR-2016, sequence version 1.
DT   27-MAR-2024, entry version 28.
DE   SubName: Full=2,5-diketo-D-gluconic acid reductase {ECO:0000313|EMBL:KUM68210.1};
GN   ORFNames=AQI70_34015 {ECO:0000313|EMBL:KUM68210.1};
OS   Streptomyces curacoi.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=146536 {ECO:0000313|EMBL:KUM68210.1, ECO:0000313|Proteomes:UP000054024};
RN   [1] {ECO:0000313|EMBL:KUM68210.1, ECO:0000313|Proteomes:UP000054024}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 40107 {ECO:0000313|EMBL:KUM68210.1,
RC   ECO:0000313|Proteomes:UP000054024};
RA   Ruckert C., Winkler A., Kalinowski J., Kampfer P., Glaeser S.;
RT   "Draft genome sequence of Streptomyces curacoi DSM 40107, type strain for
RT   the species Streptomyces curacoi.";
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KUM68210.1}.
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DR   EMBL; LMWJ01000032; KUM68210.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A124GUV9; -.
DR   STRING; 146536.AQI70_34015; -.
DR   Proteomes; UP000054024; Unassembled WGS sequence.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR   GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR   CDD; cd19133; AKR_AKR5F1; 1.
DR   Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR   PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 2.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000054024}.
FT   DOMAIN          33..268
FT                   /note="NADP-dependent oxidoreductase"
FT                   /evidence="ECO:0000259|Pfam:PF00248"
FT   ACT_SITE        60
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT   BINDING         118
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT   SITE            85
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ   SEQUENCE   293 AA;  33121 MW;  147EFCAB00268BEB CRC64;
     MRQKDVPTVS QNIPNVTLKN GVQMPILGFG VFQVPAEETE RTVTEALAAG YRHIDTAQGY
     QNEEAVGRVI TKSGIPRDEL FITTKLWIQD AGEERTKRAF DTSLRKLGLD HLDLYLIHQP
     FGDIYGSWRA MQDLYRQGAI RAIGVSNFLP DRLIDLIDHN EITPAVNQIE THPFYQRTLD
     QEIMDKRGVR LESWGPLGQG RDDLFTNPVL SGIASAHGKT VAQTVLRWLV QRNIMVIPKS
     ARPERMAENL DIFDFTLSSE EMARIATLDT GASSVVDYHD PDTVAWMGKR RFD
//