ID A0A125QAK7_9BRAD Unreviewed; 423 AA.
AC A0A125QAK7;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Ribosomal large subunit pseudouridine synthase C {ECO:0000256|ARBA:ARBA00017128};
DE EC=5.4.99.24 {ECO:0000256|ARBA:ARBA00012785};
DE AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase {ECO:0000256|ARBA:ARBA00030705};
DE AltName: Full=rRNA pseudouridylate synthase C {ECO:0000256|ARBA:ARBA00031975};
DE AltName: Full=rRNA-uridine isomerase C {ECO:0000256|ARBA:ARBA00033053};
GN ORFNames=AS156_27465 {ECO:0000313|EMBL:KWV60846.1};
OS Bradyrhizobium macuxiense.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=1755647 {ECO:0000313|EMBL:KWV60846.1, ECO:0000313|Proteomes:UP000057737};
RN [1] {ECO:0000313|EMBL:KWV60846.1, ECO:0000313|Proteomes:UP000057737}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BR 10303 {ECO:0000313|EMBL:KWV60846.1,
RC ECO:0000313|Proteomes:UP000057737};
RA Zelli J.E., Simoes-Araujo J.L., Barauna A.C., Silva K.;
RT "Draft Genome Sequence of the Strain BR 10303 (Bradyrhizobium sp.) isolated
RT from nodules of Centrolobium paraense.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC positions 955, 2504 and 2580 in 23S ribosomal RNA.
CC {ECO:0000256|ARBA:ARBA00002876}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(955/2504/2580) in 23S rRNA =
CC pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC ChEBI:CHEBI:65315; EC=5.4.99.24;
CC Evidence={ECO:0000256|ARBA:ARBA00000381};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KWV60846.1}.
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DR EMBL; LNCU01000011; KWV60846.1; -; Genomic_DNA.
DR RefSeq; WP_066499624.1; NZ_LNCU01000011.1.
DR AlphaFoldDB; A0A125QAK7; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000057737; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF93; PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 109..165
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT REGION 1..92
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 236
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 423 AA; 46935 MW; 76369282A082E8EB CRC64;
MSRRIKRTIS PGDRAKERTK GGPKGGPKSA SKGGSKSGAG HRSRKPEDRK ASDRPSSHRA
SGKPPRFAEP RREPRKRPEP VAATPKAANE LPLPTKVQTV VVTADEDNMR VDRFLEARFP
GLSFSHIQRI VRKGELRVNG RRADSKDRLE EGQSVRIPPL RLDAPKTVGV LSEAQTKTLQ
ALKEMTLYED DDVLVLNKPA GLAVQGGSGM TRHVDQMLEV MRDAKGQKPR LVHRIDRETS
GCLLIAKTRF AASHLTGAFR HRSARKIYWA LVAGVPKPKQ GRISTYLAKE ENEEDTIMRI
AEHGDEGASH AVTYYAVVEA SATKLAWVSL KPVTGRTHQL RAHMAHIDHA IVGDPKYFNK
ENWQLPGGLQ NRLHLLARRI VIPHPRGGVI DVSAPLPPHM QQSWNLLGLE ADRFDPIENA
PEE
//
