ID A0A125Y3J3_AERCA Unreviewed; 488 AA.
AC A0A125Y3J3;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Tryptophanase {ECO:0000256|HAMAP-Rule:MF_00544};
DE EC=4.1.99.1 {ECO:0000256|HAMAP-Rule:MF_00544};
DE AltName: Full=L-tryptophan indole-lyase {ECO:0000256|HAMAP-Rule:MF_00544};
DE Short=TNase {ECO:0000256|HAMAP-Rule:MF_00544};
GN Name=tnaA {ECO:0000256|HAMAP-Rule:MF_00544,
GN ECO:0000313|EMBL:NKD18370.1};
GN ORFNames=C0708_17275 {ECO:0000313|EMBL:AXB10060.1}, C1C91_08870
GN {ECO:0000313|EMBL:AXB05090.1}, DN604_09375
GN {ECO:0000313|EMBL:RWT76647.1}, HED45_20145
GN {ECO:0000313|EMBL:NKD18370.1}, KAM348_30030
GN {ECO:0000313|EMBL:GJA55580.1}, KAM379_27920
GN {ECO:0000313|EMBL:GJB73734.1}, N5I07_21750
GN {ECO:0000313|EMBL:MDH1900123.1}, N5I20_18000
GN {ECO:0000313|EMBL:MDH1506946.1}, WP2W18E01_27040
GN {ECO:0000313|EMBL:BBQ31122.1};
OS Aeromonas caviae (Aeromonas punctata).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
OC Aeromonadaceae; Aeromonas.
OX NCBI_TaxID=648 {ECO:0000313|EMBL:NKD18370.1, ECO:0000313|Proteomes:UP000705611};
RN [1] {ECO:0000313|EMBL:RWT76647.1, ECO:0000313|Proteomes:UP000288891}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GEO_23_Down_B {ECO:0000313|EMBL:RWT76647.1,
RC ECO:0000313|Proteomes:UP000288891};
RA Mathys D.A., Mollenkopf D.F., Feicht S.M., Adams R.J., Albers A.L.,
RA Stuever D.M., Daniels J.B., Wittum T.E.;
RT "Carbapenemase-producing Enterobacteriaceae present in wastewater treatment
RT plant effluent and nearby surface waters in the US.";
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000266772, ECO:0000313|Proteomes:UP000266778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R25-2 {ECO:0000313|Proteomes:UP000266772}, and T25-39
RC {ECO:0000313|Proteomes:UP000266778};
RA Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT "Genetic characterization and potential molecular dissemination mechanism
RT of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT treatment system.";
RL J Environ 76:259-266(2019).
RN [3] {ECO:0000313|EMBL:AXB05090.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=R25-2 {ECO:0000313|EMBL:AXB10060.1}, and T25-39
RC {ECO:0000313|EMBL:AXB05090.1};
RX PubMed=30528016; DOI=10.3791/1549;
RA Shi Y., Tian Z., Leclercq S.O., Zhang H., Yang M., Zhang Y.;
RT "Genetic characterization and potential molecular dissemination mechanism
RT of tet (31) gene in Aeromonas caviae from an oxytetracycline wastewater
RT treatment system.";
RL J Environ 76:259-266(2019).
RN [4] {ECO:0000313|EMBL:BBQ31122.1, ECO:0000313|Proteomes:UP000515756}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=WP2-W18-ESBL-01 {ECO:0000313|EMBL:BBQ31122.1,
RC ECO:0000313|Proteomes:UP000515756};
RA Sekizuka T., Itokawa K., Yatsu K., Inamine Y., Kuroda M.;
RT "complete genome sequences of Aeromonas caviae str. WP2-W18-ESBL-01
RT isolated from wastewater treatment plant effluent.";
RL Submitted (DEC-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:NKD18370.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UFMG-H8 {ECO:0000313|EMBL:NKD18370.1};
RA Giannattasio-Ferraz S., Maskeri L., Penido A., Barbosa-Stancioli E.F.,
RA Putonti C.;
RT "Bacterial samples isolated from urine from healthy bovine heifers (Gyr
RT breed).";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:GJA55580.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=KAM348 {ECO:0000313|EMBL:GJA55580.1}, and KAM379
RC {ECO:0000313|EMBL:GJB73734.1};
RA Maehana S., Suzuki M., Kitasato H.;
RT "Draft genome sequence of carbapenem-resistant Aeromonas spp. in Japan.";
RL Submitted (JUL-2021) to the EMBL/GenBank/DDBJ databases.
RN [7] {ECO:0000313|EMBL:MDH1506946.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GD03710 {ECO:0000313|EMBL:MDH1506946.1}, and GD03796
RC {ECO:0000313|EMBL:MDH1900123.1};
RA Diorio-Toth L.;
RT "Intensive care unit water sources are persistently colonized with multi-
RT drug resistant bacteria and are the site of extensive horizontal gene
RT transfer of antibiotic resistance genes.";
RL Submitted (SEP-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-tryptophan = indole + NH4(+) + pyruvate;
CC Xref=Rhea:RHEA:19553, ChEBI:CHEBI:15361, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:16881, ChEBI:CHEBI:28938, ChEBI:CHEBI:57912; EC=4.1.99.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001826, ECO:0000256|HAMAP-
CC Rule:MF_00544};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000256|ARBA:ARBA00001933,
CC ECO:0000256|HAMAP-Rule:MF_00544, ECO:0000256|PIRSR:PIRSR611166-50};
CC -!- PATHWAY: Amino-acid degradation; L-tryptophan degradation via pyruvate
CC pathway; indole and pyruvate from L-tryptophan: step 1/1.
CC {ECO:0000256|ARBA:ARBA00004662, ECO:0000256|HAMAP-Rule:MF_00544}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881,
CC ECO:0000256|HAMAP-Rule:MF_00544}.
CC -!- SIMILARITY: Belongs to the beta-eliminating lyase family.
CC {ECO:0000256|ARBA:ARBA00009721, ECO:0000256|HAMAP-Rule:MF_00544}.
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DR EMBL; CP025706; AXB05090.1; -; Genomic_DNA.
DR EMBL; CP025777; AXB10060.1; -; Genomic_DNA.
DR EMBL; AP021927; BBQ31122.1; -; Genomic_DNA.
DR EMBL; BPNL01000037; GJA55580.1; -; Genomic_DNA.
DR EMBL; BPOM01000023; GJB73734.1; -; Genomic_DNA.
DR EMBL; JAOCIZ010000088; MDH1506946.1; -; Genomic_DNA.
DR EMBL; JAOCFT010000001; MDH1900123.1; -; Genomic_DNA.
DR EMBL; JAAVMO010000016; NKD18370.1; -; Genomic_DNA.
DR EMBL; QKPH01000024; RWT76647.1; -; Genomic_DNA.
DR RefSeq; WP_010672391.1; NZ_WUTZ01000161.1.
DR GeneID; 70085490; -.
DR KEGG; acav:VI35_20810; -.
DR OrthoDB; 9764079at2; -.
DR UniPathway; UPA00332; UER00452.
DR Proteomes; UP000266772; Chromosome.
DR Proteomes; UP000266778; Chromosome.
DR Proteomes; UP000288891; Unassembled WGS sequence.
DR Proteomes; UP000515756; Chromosome.
DR Proteomes; UP000705611; Unassembled WGS sequence.
DR Proteomes; UP000887009; Unassembled WGS sequence.
DR Proteomes; UP000887145; Unassembled WGS sequence.
DR Proteomes; UP001160758; Unassembled WGS sequence.
DR Proteomes; UP001161704; Unassembled WGS sequence.
DR GO; GO:0009034; F:tryptophanase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.1150.10; Aspartate Aminotransferase, domain 1; 1.
DR Gene3D; 3.40.640.10; Type I PLP-dependent aspartate aminotransferase-like (Major domain); 1.
DR HAMAP; MF_00544; Tryptophanase; 1.
DR InterPro; IPR001597; ArAA_b-elim_lyase/Thr_aldolase.
DR InterPro; IPR011166; Beta-eliminating_lyase.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR013440; TNase.
DR InterPro; IPR018176; Tryptophanase_CS.
DR NCBIfam; TIGR02617; tnaA_trp_ase; 1.
DR PANTHER; PTHR32325; BETA-ELIMINATING LYASE-LIKE PROTEIN-RELATED; 1.
DR PANTHER; PTHR32325:SF4; TRYPTOPHANASE; 1.
DR Pfam; PF01212; Beta_elim_lyase; 1.
DR PIRSF; PIRSF001386; Trpase; 1.
DR SUPFAM; SSF53383; PLP-dependent transferases; 1.
DR PROSITE; PS00853; BETA_ELIM_LYASE; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|HAMAP-Rule:MF_00544, ECO:0000313|EMBL:NKD18370.1};
KW Pyridoxal phosphate {ECO:0000256|ARBA:ARBA00022898, ECO:0000256|HAMAP-
KW Rule:MF_00544};
KW Tryptophan catabolism {ECO:0000256|ARBA:ARBA00023079, ECO:0000256|HAMAP-
KW Rule:MF_00544}.
FT DOMAIN 49..437
FT /note="Aromatic amino acid beta-eliminating lyase/threonine
FT aldolase"
FT /evidence="ECO:0000259|Pfam:PF01212"
FT MOD_RES 270
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00544,
FT ECO:0000256|PIRSR:PIRSR611166-50"
SQ SEQUENCE 488 AA; 54263 MW; AEF19002211CB045 CRC64;
MENFKHLPEP FRIRVIEPVK RTTRAYREEA IVRAGMNPFL LDSEDVFIDL LTDSGTGAIT
QDMQAAMLRG DEAYSGSRSY YALQQAVQEI FGYSLTIPTH QGRGAEQIYI PVLIKKREQE
KGLDRKKMVA LSNYFFDTTQ GHTQINCCVA KNVYTREAFD TSVTADFKGN FDLVKLEEAI
LEAGPANVPY IVSTITCNSA GGQPVSIANL KAVYEIARKY DIPVIMDSAR FAENAYFIQQ
REPGYANWTI EEITRESYKY ADGLAMSAKK DAMVQMGGLL CFKDDSMLDV YTECRTLCVV
QEGFPTYGGL EGGAMERLAV GLRDGMRQDW LAYRIGQVQY LVDGLEAIGV VCQQAGGHAA
FVDAGKLLPH IPADQFPAHA LACELYKVAG IRAVEIGSLL LGRDPATGKQ HPCPAELLRL
TIPRATYTQT HMDFVIEAFG EVKKNAHRVK GLDFIYEPAV LRHFTARLKE VEHNDPSAGK
TQKKLIEA
//
