ID A0A127VEI4_9SPHI Unreviewed; 480 AA.
AC A0A127VEI4;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Probable membrane transporter protein {ECO:0000256|RuleBase:RU363041};
GN ORFNames=AY601_2448 {ECO:0000313|EMBL:AMP99338.1};
OS Pedobacter cryoconitis.
OC Bacteria; Bacteroidota; Sphingobacteriia; Sphingobacteriales;
OC Sphingobacteriaceae; Pedobacter.
OX NCBI_TaxID=188932 {ECO:0000313|EMBL:AMP99338.1, ECO:0000313|Proteomes:UP000071561};
RN [1] {ECO:0000313|EMBL:AMP99338.1, ECO:0000313|Proteomes:UP000071561}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PAMC 27485 {ECO:0000313|EMBL:AMP99338.1,
RC ECO:0000313|Proteomes:UP000071561};
RA Lee J., Kim O.-S.;
RT "Complete genome sequence of Pedobacter cryoconitis PAMC 27485.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + precorrin-2 = 2 H(+) + NADH + sirohydrochlorin;
CC Xref=Rhea:RHEA:15613, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58351, ChEBI:CHEBI:58827; EC=1.3.1.76;
CC Evidence={ECO:0000256|ARBA:ARBA00001156};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; siroheme
CC biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
CC {ECO:0000256|ARBA:ARBA00005010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU363041};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU363041}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the 4-toluene sulfonate uptake permease (TSUP)
CC (TC 2.A.102) family. {ECO:0000256|RuleBase:RU363041}.
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DR EMBL; CP014504; AMP99338.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A127VEI4; -.
DR KEGG; pcm:AY601_2448; -.
DR PATRIC; fig|188932.3.peg.2558; -.
DR UniPathway; UPA00262; UER00222.
DR Proteomes; UP000071561; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004325; F:ferrochelatase activity; IEA:InterPro.
DR GO; GO:0043115; F:precorrin-2 dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019354; P:siroheme biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR028161; Met8-like.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR028281; Sirohaem_synthase_central.
DR InterPro; IPR006367; Sirohaem_synthase_N.
DR InterPro; IPR002781; TM_pro_TauE-like.
DR NCBIfam; TIGR01470; cysG_Nterm; 1.
DR PANTHER; PTHR35330; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR PANTHER; PTHR35330:SF1; SIROHEME BIOSYNTHESIS PROTEIN MET8; 1.
DR Pfam; PF13241; NAD_binding_7; 1.
DR Pfam; PF14824; Sirohm_synth_M; 1.
DR Pfam; PF01925; TauE; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF75615; Siroheme synthase middle domains-like; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU363041};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU363041};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Porphyrin biosynthesis {ECO:0000256|ARBA:ARBA00023244};
KW Reference proteome {ECO:0000313|Proteomes:UP000071561};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU363041};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU363041}.
FT TRANSMEM 197..216
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 231..255
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 300..319
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 365..393
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 405..422
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 429..445
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU363041"
FT DOMAIN 111..132
FT /note="Siroheme synthase central"
FT /evidence="ECO:0000259|Pfam:PF14824"
SQ SEQUENCE 480 AA; 51791 MW; E8D347E08D808DE7 CRC64;
MHTVIIGAGP VGLEKLNAVL GQSEQAKITV VAEEIILEVY ELAERLTQVK LLKKSYTAAD
LHQADIVIAA TNNNGLNEEI IRDAHALKLL VNVADKPDLC DFYLGSIVKK GDLKIAISTN
GKSPTIAKRL KELFNDNLPG ELDITLQQMS SFRNTLQGDF SSKVKKLNEV SAVLVEPAVQ
EFAEPAVQKT SYPKFKWLIW LTIVLSIAVL IAVFWQREPE FQAYVAGINP MFYWFLAGGF
IFAMIDGAIG MSYGVTSTAF SLSMGIPPAS ASMGVHLSEV LSNGIAGWMH YRMGNINWKL
FKLLIIPGIL GAVAGAYLLS SLEHYGAYIK PFISLYTLIL GGVILSKAFK VSRKRVGGKI
KKITLLGLFG GFIDAVGGGG WGSIVLSSLI AGGRNARFSL GTVKITRFFI ALMSSLTFIT
MLKSAHWEAV AGLVIGSALA APIAAKVSNR ISVKSIMVAV GIIVIAVSLR SIIMFILKVI
//
