ID A0A133UPN1_9EURY Unreviewed; 447 AA.
AC A0A133UPN1;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KXA96184.1};
GN ORFNames=AKJ38_03740 {ECO:0000313|EMBL:KXA96184.1};
OS candidate division MSBL1 archaeon SCGC-AAA259I14.
OC Archaea; Euryarchaeota; candidate division MSBL1.
OX NCBI_TaxID=1698268 {ECO:0000313|EMBL:KXA96184.1, ECO:0000313|Proteomes:UP000070414};
RN [1] {ECO:0000313|EMBL:KXA96184.1, ECO:0000313|Proteomes:UP000070414}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCGC-AAA259I14 {ECO:0000313|EMBL:KXA96184.1};
RX PubMed=26758088; DOI=10.1038/srep19181;
RA Mwirichia R., Alam I., Rashid M., Vinu M., Ba-Alawi W., Anthony Kamau A.,
RA Kamanda Ngugi D., Goker M., Klenk H.P., Bajic V., Stingl U.;
RT "Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine
RT pools of the Red Sea.";
RL Sci. Rep. 6:19181-19181(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXA96184.1}.
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DR EMBL; LHXS01000083; KXA96184.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A133UPN1; -.
DR PATRIC; fig|1698268.3.peg.973; -.
DR Proteomes; UP000070414; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000070414}.
FT DOMAIN 9..142
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 181..406
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 447 AA; 50833 MW; 1B49C1697F058E1A CRC64;
MRWLLVEPPV QSDIQASLGA VGIPSSAYLA PILERNNEEV RIEDAPALDH NLEDVADVIR
DYDPDYVGIS ALTTDIEEAY RVAEAAKKSD QSRKVILGGA HPTLRPKETL EECSSVDIVA
RGEGEEIIDE ISRFEDLSRI KGISYRENGN IIENQDREPI MNLDSLPFPA YHLLPMERYE
FDGIRYATMI TSRGCPFNCV FCASSRICGK KWRGKSPERV LEQMKALRYD YDVKEIEILD
DTFVLDKNRA TEICDLMVEE GLDITWSCSS RVDTMDSSLA EKLREAGCHT VYMGIESGVQ
EVLDRLKKGI TIEQARDAVE AVKEADMNVV GSFILGVPGE TKKQMEKTID FAKELDITLA
QFTLFTPYPG TESYENAEEN DLFLTKDWSD FSTLDPIIKN DEMSAEELKN FMRQAYIRFY
LRPSYIWRAV KTGELWSLLK KGISWFI
//
