ID A0A133V924_9EURY Unreviewed; 447 AA.
AC A0A133V924;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:KXB02897.1};
GN ORFNames=AKJ43_00080 {ECO:0000313|EMBL:KXB02897.1};
OS candidate division MSBL1 archaeon SCGC-AAA261D19.
OC Archaea; Euryarchaeota; candidate division MSBL1.
OX NCBI_TaxID=1698273 {ECO:0000313|EMBL:KXB02897.1, ECO:0000313|Proteomes:UP000070400};
RN [1] {ECO:0000313|EMBL:KXB02897.1, ECO:0000313|Proteomes:UP000070400}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCGC-AAA261D19 {ECO:0000313|EMBL:KXB02897.1};
RX PubMed=26758088; DOI=10.1038/srep19181;
RA Mwirichia R., Alam I., Rashid M., Vinu M., Ba-Alawi W., Anthony Kamau A.,
RA Kamanda Ngugi D., Goker M., Klenk H.P., Bajic V., Stingl U.;
RT "Metabolic traits of an uncultured archaeal lineage -MSBL1- from brine
RT pools of the Red Sea.";
RL Sci. Rep. 6:19181-19181(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXB02897.1}.
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DR EMBL; LHXX01000001; KXB02897.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A133V924; -.
DR PATRIC; fig|1698273.3.peg.16; -.
DR Proteomes; UP000070400; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485};
KW Reference proteome {ECO:0000313|Proteomes:UP000070400}.
FT DOMAIN 7..142
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 181..406
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 447 AA; 50372 MW; 58EA4715E1C28782 CRC64;
MKAMFVDPPV QSAIQSRLGA TGIPSSAYLA PILEEEGHEV KIVDTVTMGY DLEDVKAEIK
RFDPELLGAT SVTPTIYSTY EIAKIAKELN SDCITVVGGP HPTFAAKRIV EECPYIDVAV
RGEGEETLKE LVSTKELDDI KGITYRKCDR IVENKSRPFI QDLDSLPFPA YHLLPMDKYE
LGGFKYATMM TSRGCPFNCI FCSSSGICGK AWRGNSPDRV LEQIKLLVSE YEISEIEMLD
DTFLFDNKRA RNICDLIIEE GLDVSWSCSS RVDTITKPLA EKLKEAGCHT IYMGIESGSQ
KILNRLRKGV SIEQIKKAVK IVKEAGLDVF GSFVLGVPGE TKRLMEKTVD FAKKLRLTFA
QFTLFTPYPG TEAYEIAERD DLFLTKDWSQ YTTLDPVMKI SKMTAEEIKG FLTKAYLSFY
LRPSYIWDSI KGRRFWGFLK ALLNKKV
//
