ID A0A133YGZ9_9FIRM Unreviewed; 550 AA.
AC A0A133YGZ9;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000256|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000256|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000256|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000256|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000256|HAMAP-Rule:MF_01543};
GN ORFNames=HMPREF1872_00137 {ECO:0000313|EMBL:KXB42465.1};
OS Amygdalobacter nucleatus.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Amygdalobacter.
OX NCBI_TaxID=3029274 {ECO:0000313|EMBL:KXB42465.1, ECO:0000313|Proteomes:UP000070080};
RN [1] {ECO:0000313|Proteomes:UP000070080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KA00274 {ECO:0000313|Proteomes:UP000070080};
RA Mitreva M., Pepin K.H., Mihindukulasuriya K.A., Fulton R., Fronick C.,
RA O'Laughlin M., Miner T., Herter B., Rosa B.A., Cordes M., Tomlinson C.,
RA Wollam A., Palsikar V.B., Mardis E.R., Wilson R.K.;
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC EC=6.3.4.3; Evidence={ECO:0000256|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000256|HAMAP-Rule:MF_01543}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXB42465.1}.
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DR EMBL; LSCV01000002; KXB42465.1; -; Genomic_DNA.
DR RefSeq; WP_066712452.1; NZ_KQ959565.1.
DR AlphaFoldDB; A0A133YGZ9; -.
DR STRING; 1497955.HMPREF1872_00137; -.
DR PATRIC; fig|1497955.3.peg.136; -.
DR OrthoDB; 9761733at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000070080; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW Ligase {ECO:0000256|HAMAP-Rule:MF_01543, ECO:0000313|EMBL:KXB42465.1};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_01543};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563, ECO:0000256|HAMAP-
KW Rule:MF_01543}; Reference proteome {ECO:0000313|Proteomes:UP000070080}.
FT BINDING 55..62
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01543"
SQ SEQUENCE 550 AA; 59585 MW; ECBA0A031A34CE21 CRC64;
MSDCKLEPIT KVAAKLGLTS ENVYAYGPYK AKLPLSLMRK NPKGKLILVT ATSPTPLGEG
KTTMAIGLAD AMAKLGKSVC AALREPSMGP TFGLKGGACG GGQAKIVPED EINLHFTGDF
AAITQANNLI AACLDNHIYW GNELQIDPAK IVFKRALDLN ERSLRNFNYL IKQPKSVENQ
QTYNVSGQFN ITAASELMAI FGLASDFKDL RQRLDNILLA FNFNSEPVYL RSLKITDALL
LILKDAFNPN LVQTAKHNPA IVHGGAFANI ANGTNSFVST KLACSLADYV VTEAGFGADL
GLEKYFDLLA GPRQLKVDCV VLVTSLRALK YQAGLALKDT TKENLYALET GLANLKRHMQ
IIQNFQVPFV VCLNKFANDT ETEINFLKAK LNEQGLTLAV ADVFSQDSEG ADDLAHKVIQ
KIVSEANVAN AKQLFSENAD LMTKLTCIAK QIYGAKDVSL SEEAEADLAL IRQQGWESMS
LCIAKTPSSF TDQANLLNAP TDFTLHVTSF SAYRGANFIV AHCGKVLTMP GLPKKPAACE
MICSDAEINL
//
