ID A0A136PQ49_9ACTN Unreviewed; 2252 AA.
AC A0A136PQ49;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 32.
DE SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:KXK60531.1};
GN ORFNames=AWW66_18410 {ECO:0000313|EMBL:KXK60531.1};
OS Micromonospora rosaria.
OC Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC Micromonosporaceae; Micromonospora.
OX NCBI_TaxID=47874 {ECO:0000313|EMBL:KXK60531.1, ECO:0000313|Proteomes:UP000070620};
RN [1] {ECO:0000313|EMBL:KXK60531.1, ECO:0000313|Proteomes:UP000070620}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 803 {ECO:0000313|EMBL:KXK60531.1,
RC ECO:0000313|Proteomes:UP000070620};
RA Yang H., He X., Zhu D.;
RT "Whole genome sequence and analysis of Micromonospora rosaria DSM 803,
RT which can produce antibacterial substance rosamicin.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXK60531.1}.
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DR EMBL; LRQV01000068; KXK60531.1; -; Genomic_DNA.
DR RefSeq; WP_067367782.1; NZ_LRQV01000068.1.
DR OrthoDB; 5478077at2; -.
DR Proteomes; UP000070620; Unassembled WGS sequence.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd12117; A_NRPS_Srf_like; 1.
DR CDD; cd19531; LCL_NRPS-like; 1.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 1.10.1240.100; -; 1.
DR Gene3D; 3.30.300.30; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.40.50.980; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 2.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 1.
DR PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR Pfam; PF00501; AMP-binding; 1.
DR Pfam; PF00668; Condensation; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF00550; PP-binding; 2.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 2.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR SUPFAM; SSF47336; ACP-like; 2.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR SUPFAM; SSF53901; Thiolase-like; 1.
DR PROSITE; PS00455; AMP_BINDING; 1.
DR PROSITE; PS50075; CARRIER; 2.
DR PROSITE; PS00606; KS3_1; 1.
DR PROSITE; PS52004; KS3_2; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000070620};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 44..460
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 656..731
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1714..1788
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 2252 AA; 243284 MW; 1044AEB29BD09B55 CRC64;
MRSNAELKKD LLRYLLMQVK QEALDVDRAK EFIRAIGADG RDADDRIAVV GMACRFPGAS
DKEQFWRNLV DGRESIGDFP PLRLDDLRRV EGGTSAVRKG GYLDRIDLFD AEYFGIPPHV
ATQLDPYHRN LLEVLVETME DAGYARSELS GSRTGIFVGN DHTHRLITSY LPFLSELDFA
AITGSWSGIL ASRLSYLFDL RGPATVIDTG CSSGLVAVDA AIKALRNGDC DTAFVAAINL
FLSPSSLGNE TESADHRVRA FDTAADGTVW SEGVAGVYLK PLSRALADGD HVYGVLLGSA
VNNDGRSNGL TAPNAQAQKN LLVSAWKRAG IAPESVSYIE AHGTGTTLGD PIEVKGLTSA
FAEFTHRRQF CAIGSVKTNI GHTVGAAGLA SLIKTLMCLD RRTIPPSIHF DVPNELLDLA
NSPVYVADRL TEWEEGDTPR RAGVSSFSLS GTNCHVVLEE APARAAVPEQ ARQLRVFPIS
ARSEDLLART AALHLAHLDR HPEHRLADVC FTMQVGREHQ AVRAVVLCET RAGLRDGLLR
LLRPEQADDS PPVDDGVVLR ADAPSRPGAG LSGHLRDAVT AFLSGRARPF AGLDRDADVR
RVPLPPQPFQ HVRLWDETVR AHPVPGEPGQ EPTDPVDRLR AILSRPSHLD GLQESGADAV
PRKVIAGVWS GILGYPTIRG VDDFFALGGD SISSVKITQL LNAVLGIEIP PTTLLTKPTF
DEFADVVIRA HGVDESLVRS RLSGSTRRPV PVEHVVESYE LPLTAAQRSM FVSSQVDEES
VAYNVSGLTI RPGALDVAEL EEWLRVLVRR HDSLRSTFHL RDGEPFQRVH AEVPVAVEHR
RLGALPEGDS HESAARREMQ RFVRPFLLDS APLFRVGYFE FDDGVSCVAI DIHHIITDGT
SMGILFRDLG AIAEGAPLPP LPSSYRAAIR SLLERQGGAD MVAHRDYWVA RFADGAPTLQ
LTTDWQRAEV ATAAGATLFA SVDGETLEAA KRYARAHNLT LYMVLLGVFQ QLLSRLSGQG
DVVIGAPVMG RPDLTYQDLV GMFVTTLPIR VTAAPTTGVG DFLAGLRTTV LEAFEHQEYP
LESLVEELNP PRVPGRRPLF DVCFVHQNTD MGLEPDDERI LPFDDGSAKY DITLSTREAD
GALLLEWEYS TALFREETMA LYAERYATLL RSFLAAAVDT PVGTLDLMPE REAELVRTFA
TVPAPALDSR GIARLFEEHV AAAPDRTALI ADDRSLTYGE LNARANRIAH GLIRLGVTPG
SPVALLLDRS TDMVAAILGV LKAGCFYLPL NTDFPPERLR MMVADAGAGL LLTSDARSGQ
AAELISERLR IVGVHHLAEA TAASGNPGLP GSSADEAYIM YTSGTSGVPK GSVIRQRSVL
RVVHQSVFYA ADPSDVFLMI SEYSFDGSIY DMFGALTNGA SLVLLDKASV LDLDRLGAAI
ERHGVTSFFI TTAMFNALID HASGSLGGVR RVIFGGEIAS PAHVKRAFDL LGPGRISHAY
GPTETTVFAT IHTLDALDPD DAVPIGTAIN DTSLWVLDER LEPVPVGVSG ELYIGGAGLS
DGYLNQPELT AERFVYSPAV PGERLYKTGD IVTFKTNGLL YYTGRVDQQI KLRGYRIELA
EIIQVALDEP HVRWAHAGLY ETAEGSRSLC LWVGYVDDQD HEPVLRAALS RRLPDYMVPS
FIVKADTVPL NASGKVDVAA LPKPDFTAGP AAAAPETDSL RVLADAWQHV LGVPVDDIDA
DFFALGGDSI KAIQIVARLR EQDVAIRAAD LLGNATIRTL EEKLSGAAAT GTALRAYDPT
PRTGAITPSP IQLAFLDDPD APTRVFNQCL LVTSAQPVSL EQLVRAGERL VRHHDLLRVE
VDDQGGLSLR DVDAPTLVHA EQAPDGLSDD ALAEYLKTVQ ARVDVRGGPV VSLAAGLGDG
GERFAVAIHH LAVDVVSWGV LLEDLAISLA DPDAELPAKT MPFPAWTSEV KQRAQDGGFR
TQLPYWLDLA RGAGDAGEVF AERDVKRSET AATVVRLAET DDFSVFDAAR EAHGASHADT
VLTVVARALA SVTGQQRVLF TLEGHGREPI AGDHDLTRTV GWFTSTFPHL VQVERSVADT
MDGLRQSFDR LPDKGAGFGP LLRFDVGLGA QRSTLAAIRP QIGFNYLGGQ VSGDGITVTH
LPVDITTDGD HHLPHPLDII ASRSGGELLI EVRYPRAWEA SGTAKKIVAA VEESFHEVRN
ALKSGEPRGF QVSSPIRQDV LADILLDVGG GI
//
