ID A0A140KPT9_WHEAT Unreviewed; 353 AA.
AC A0A140KPT9;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE SubName: Full=GA2ox-B2 protein {ECO:0000313|EMBL:CFV04430.1, ECO:0000313|EnsemblPlants:TraesCS7B02G349700.1};
GN Name=GA2ox-B2 {ECO:0000313|EMBL:CFV04430.1};
GN ORFNames=CFC21_104651 {ECO:0000313|EMBL:KAF7103686.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EMBL:CFV04430.1};
RN [1] {ECO:0000313|EMBL:CFV04430.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Chinese Spring {ECO:0000313|EMBL:CFV04430.1};
RA Chooi Y.-H.;
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:CFV04430.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Chinese Spring {ECO:0000313|EMBL:CFV04430.1};
RA Phillips A.L., Huttly A.K., Pearce S.P.;
RT "Heterologous expression and transcript analysis of gibberellin
RT biosynthetic genes of grasses reveals novel functionality in the GA3ox
RT family.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAF7103686.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7103686.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [4] {ECO:0000313|EnsemblPlants:TraesCS7B02G349700.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS7B02G349700.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [5] {ECO:0000313|EnsemblPlants:TraesCS7B02G349700.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [6] {ECO:0000313|EMBL:KAF7103686.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7103686.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. {ECO:0000256|RuleBase:RU003682}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LN828677; CFV04430.1; -; Genomic_DNA.
DR EMBL; CM022230; KAF7103686.1; -; Genomic_DNA.
DR SMR; A0A140KPT9; -.
DR STRING; 4565.A0A140KPT9; -.
DR EnsemblPlants; TraesCS7B02G349700.1; TraesCS7B02G349700.1; TraesCS7B02G349700.
DR Gramene; TraesCS7B02G349700.1; TraesCS7B02G349700.1; TraesCS7B02G349700.
DR Gramene; TraesCS7B03G0937700.1; TraesCS7B03G0937700.1.CDS; TraesCS7B03G0937700.
DR Gramene; TraesWEE_scaffold_037439_01G000100.1; TraesWEE_scaffold_037439_01G000100.1; TraesWEE_scaffold_037439_01G000100.
DR OMA; WLQDKYA; -.
DR OrthoDB; 1057251at2759; -.
DR Proteomes; UP000019116; Chromosome 7B.
DR Proteomes; UP000815260; Chromosome 7B.
DR GO; GO:0052634; F:C-19 gibberellin 2-beta-dioxygenase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0045487; P:gibberellin catabolic process; IBA:GO_Central.
DR GO; GO:0009416; P:response to light stimulus; IBA:GO_Central.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR47990; 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47990:SF162; GIBBERELLIN 2-BETA-DIOXYGENASE 4; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003682};
KW Metal-binding {ECO:0000256|RuleBase:RU003682};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003682};
KW Reference proteome {ECO:0000313|Proteomes:UP000019116}.
FT DOMAIN 177..288
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 353 AA; 37487 MW; 36A9AC59B9BC7234 CRC64;
MVASSAAPAS SSAAPACRGM APAGIPTIDM SAPAGRAELS GQMVEAFAER GFFKAVNHGV
PPRVSARLDA ASAAFFARPA HEKQEAGPPD PLGYGSRSIG SHGDVGELEY LILHTDPEVV
ARKARAIDRD DPSRFSEAVN VYVQAVRHLA CQILDLLGEG LGLRDPASLS RLVTTTDSDS
LVRINNYPPS AASDGGVKAA SSVGFGEHSD PQILSVLRAN DVDGLQVLLP DGRGEDAWVQ
VPADPAAFFI NVGDLLQALT NGKLVSIRHR VMASTSKPRL SAIYFAAPAL HECISALPET
VTAAAPRRYR PFTWAEYKKT MYTLRLSHNR LDLFKVILVG DVGEGAGEDR SRI
//
