ID A0A143HPL0_9GAMM Unreviewed; 286 AA.
AC A0A143HPL0;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=isochorismatase {ECO:0000256|ARBA:ARBA00012100};
DE EC=3.3.2.1 {ECO:0000256|ARBA:ARBA00012100};
GN ORFNames=A3224_14750 {ECO:0000313|EMBL:AMX03675.1}, SAMN05660479_03172
GN {ECO:0000313|EMBL:SFD10551.1};
OS Microbulbifer thermotolerans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales;
OC Microbulbiferaceae; Microbulbifer.
OX NCBI_TaxID=252514 {ECO:0000313|EMBL:AMX03675.1, ECO:0000313|Proteomes:UP000076077};
RN [1] {ECO:0000313|Proteomes:UP000076077}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DAU221 {ECO:0000313|Proteomes:UP000076077};
RA Lee Y.-S., Choi Y.-L.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AMX03675.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DAU221 {ECO:0000313|EMBL:AMX03675.1};
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SFD10551.1, ECO:0000313|Proteomes:UP000181967}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 19189 {ECO:0000313|EMBL:SFD10551.1,
RC ECO:0000313|Proteomes:UP000181967};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000128};
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|PIRSR:PIRSR001111-50};
CC Note=Binds 1 phosphopantetheine covalently.
CC {ECO:0000256|PIRSR:PIRSR001111-50};
CC -!- PATHWAY: Siderophore biosynthesis. {ECO:0000256|ARBA:ARBA00004924}.
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DR EMBL; CP014864; AMX03675.1; -; Genomic_DNA.
DR EMBL; FOKT01000011; SFD10551.1; -; Genomic_DNA.
DR RefSeq; WP_067156339.1; NZ_JAPHQH010000022.1.
DR AlphaFoldDB; A0A143HPL0; -.
DR STRING; 252514.A3224_14750; -.
DR GeneID; 76609291; -.
DR KEGG; mthd:A3224_14750; -.
DR OrthoDB; 5794853at2; -.
DR Proteomes; UP000076077; Chromosome.
DR Proteomes; UP000181967; Unassembled WGS sequence.
DR GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR Gene3D; 1.10.1200.10; ACP-like; 1.
DR Gene3D; 3.40.50.850; Isochorismatase-like; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR016291; Isochorismatase.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR PANTHER; PTHR43540:SF3; ENTEROBACTIN SYNTHASE COMPONENT B; 1.
DR PANTHER; PTHR43540; PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR Pfam; PF00550; PP-binding; 1.
DR PIRSF; PIRSF001111; Isochorismatase; 1.
DR PRINTS; PR01398; ISCHRISMTASE.
DR SUPFAM; SSF47336; ACP-like; 1.
DR SUPFAM; SSF52499; Isochorismatase-like hydrolases; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lyase {ECO:0000313|EMBL:SFD10551.1};
KW Phosphopantetheine {ECO:0000256|PIRSR:PIRSR001111-50};
KW Phosphoprotein {ECO:0000256|PIRSR:PIRSR001111-50};
KW Reference proteome {ECO:0000313|Proteomes:UP000076077}.
FT DOMAIN 208..284
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT MOD_RES 245
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000256|PIRSR:PIRSR001111-50"
SQ SEQUENCE 286 AA; 32320 MW; 77E11BE93ECC9E7D CRC64;
MSIPSIRSYP MPQQMPENRV RWNLDAERAV LLIHDMQEYF LHFYGEESAL IDTLLANLIA
LRSWAWEHKV PVVYTAQPPS QTAEERALLT DLWGPGITAS DPEAAKIVTP LTPEKNDTVL
VKWRYSAFQR SHLEALMRDW GRDQLIIGGV YAHIGCMTTA LDAFMRDIKP FLVADAVADF
SPREHQQALD FVAGRCGSVT TVEEILNPVA KVDFPWLTAQ ITPLLEEDAG TPDPEDNLLD
YGLDSIQVMQ LIGRWQQLGI HLSFEELAGE PTLANWWKLI EQKQAA
//
