ID   A0A143XN64_9FIRM        Unreviewed;       240 AA.
AC   A0A143XN64;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=tRNA 2-thiocytidine biosynthesis protein TtcA {ECO:0000313|EMBL:CVI68405.1};
GN   Name=ttcA_2 {ECO:0000313|EMBL:CVI68405.1};
GN   ORFNames=NDGK_01204 {ECO:0000313|EMBL:CVI68405.1};
OS   Clostridiales bacterium CHKCI001.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales.
OX   NCBI_TaxID=1780378 {ECO:0000313|EMBL:CVI68405.1, ECO:0000313|Proteomes:UP000198774};
RN   [1] {ECO:0000313|EMBL:CVI68405.1, ECO:0000313|Proteomes:UP000198774}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CHKC1 {ECO:0000313|EMBL:CVI68405.1};
RA   Oliw E.H.;
RL   Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
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DR   EMBL; FCNS01000013; CVI68405.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A143XN64; -.
DR   STRING; 1780378.NDGK_01204; -.
DR   OrthoDB; 9801054at2; -.
DR   Proteomes; UP000198774; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006400; P:tRNA modification; IEA:UniProt.
DR   CDD; cd01993; Alpha_ANH_like_II; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR035107; tRNA_thiolation_TtcA_Ctu1.
DR   PANTHER; PTHR43686; SULFURTRANSFERASE-RELATED; 1.
DR   PANTHER; PTHR43686:SF1; TRNA-CYTIDINE(32) 2-SULFURTRANSFERASE; 1.
DR   Pfam; PF01171; ATP_bind_3; 1.
DR   PIRSF; PIRSF004976; ATPase_YdaO; 1.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|PIRSR:PIRSR004976-51};
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|PIRSR:PIRSR004976-51};
KW   Reference proteome {ECO:0000313|Proteomes:UP000198774};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          25..205
FT                   /note="tRNA(Ile)-lysidine/2-thiocytidine synthase N-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF01171"
FT   BINDING         29..31
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004976-51"
FT   BINDING         35
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004976-51"
FT   BINDING         61
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004976-51"
FT   BINDING         131
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004976-51"
FT   BINDING         136
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR004976-51"
SQ   SEQUENCE   240 AA;  27774 MW;  83D0955D17BF14FC CRC64;
     MELQKLLSYT RQAVEDYHLI EQNDKIMVGL SGGKDSIALL YALSHLQRFY PSPFSVHAVT
     VDMGFSEYDT TSLQKLCDWL QIPFTKISTE IGSIVFEERK EKNPCALCAK LRRGTLLNAA
     QELGCNKIAY GHHKDDIIET MLLSLFFEGR FYSFGPYTYF SDRKIAIIRP LIYVSESEVI
     GFMKKQNLIP IKNPCPADRH TKREEMKQLL KKLNHQYPGV KKRLFSAIQN GKIEDWDRRN
//