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Database: UniProt
Entry: A0A148N530_9GAMM
LinkDB: A0A148N530_9GAMM
Original site: A0A148N530_9GAMM 
ID   A0A148N530_9GAMM        Unreviewed;       522 AA.
AC   A0A148N530;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   23-MAY-2018, entry version 8.
DE   RecName: Full=NAD(P) transhydrogenase subunit alpha {ECO:0000256|PIRNR:PIRNR000203};
DE            EC=1.6.1.2 {ECO:0000256|PIRNR:PIRNR000203};
GN   Name=pntA {ECO:0000313|EMBL:KXJ39628.1};
GN   ORFNames=AXA67_11180 {ECO:0000313|EMBL:KXJ39628.1};
OS   Methylothermaceae bacteria B42.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales;
OC   Methylothermaceae.
OX   NCBI_TaxID=1798802 {ECO:0000313|EMBL:KXJ39628.1, ECO:0000313|Proteomes:UP000074680};
RN   [1] {ECO:0000313|EMBL:KXJ39628.1, ECO:0000313|Proteomes:UP000074680}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B42 {ECO:0000313|EMBL:KXJ39628.1};
RX   PubMed=26779119; DOI=10.3389/fmicb.2015.01425;
RA   Skennerton C.T., Ward L.M., Michel A., Metcalfe K., Valiente C.,
RA   Mullin S., Chan K.Y., Gradinaru V., Orphan V.J.;
RT   "Genomic Reconstruction of an Uncultured Hydrothermal Vent
RT   Gammaproteobacterial Methanotroph (Family Methylothermaceae) Indicates
RT   Multiple Adaptations to Oxygen Limitation.";
RL   Front. Microbiol. 6:1425-1425(2015).
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled
CC       to respiration and ATP hydrolysis and functions as a proton pump
CC       across the membrane. {ECO:0000256|PIRNR:PIRNR000203}.
CC   -!- CATALYTIC ACTIVITY: NADPH + NAD(+) = NADP(+) + NADH.
CC       {ECO:0000256|PIRNR:PIRNR000203}.
CC   -!- SIMILARITY: Belongs to the AlaDH/PNT family.
CC       {ECO:0000256|PIRNR:PIRNR000203}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:KXJ39628.1}.
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DR   EMBL; LSNW01000032; KXJ39628.1; -; Genomic_DNA.
DR   Proteomes; UP000074680; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008750; F:NAD(P)+ transhydrogenase (AB-specific) activity; IEA:UniProtKB-EC.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:InterPro.
DR   InterPro; IPR008143; Ala_DH/PNT_CS2.
DR   InterPro; IPR008142; AlaDH/PNT_CS1.
DR   InterPro; IPR007886; AlaDH/PNT_N.
DR   InterPro; IPR007698; AlaDH/PNT_NAD(H)-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR026255; NADP_transhyd_a.
DR   InterPro; IPR024605; NADP_transhyd_a_C.
DR   Pfam; PF01262; AlaDh_PNT_C; 1.
DR   Pfam; PF05222; AlaDh_PNT_N; 1.
DR   Pfam; PF12769; PNTB_4TM; 1.
DR   PIRSF; PIRSF000203; NADP_transhydrogenase_alpha; 1.
DR   SMART; SM01002; AlaDh_PNT_C; 1.
DR   SMART; SM01003; AlaDh_PNT_N; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR00561; pntA; 1.
DR   PROSITE; PS00836; ALADH_PNT_1; 1.
DR   PROSITE; PS00837; ALADH_PNT_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000074680};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   NAD {ECO:0000256|PIRNR:PIRNR000203};
KW   NADP {ECO:0000256|PIRNR:PIRNR000203};
KW   Nucleotide-binding {ECO:0000256|PIRNR:PIRNR000203};
KW   Oxidoreductase {ECO:0000256|PIRNR:PIRNR000203};
KW   Reference proteome {ECO:0000313|Proteomes:UP000074680};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    175    198       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    412    431       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    437    458       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    465    484       Helical. {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM    490    512       Helical. {ECO:0000256|SAM:Phobius}.
FT   DOMAIN        4    144       AlaDh_PNT_N. {ECO:0000259|SMART:SM01003}.
FT   DOMAIN      153    318       AlaDh_PNT_C. {ECO:0000259|SMART:SM01002}.
SQ   SEQUENCE   522 AA;  55862 MW;  95E1742014A4161B CRC64;
     MKIGVPKEVH EGERRVATTP TVAEQLQKLG FTVAIEAGAG AEANFSDDAY REVGVEIIDD
     ARKLWEASDI VLKVRPPEVH PFCGPEAELL REGGNLISFI WPAQHPELLE KFKERKATVL
     AMDCIPRISR AQKMDALSSM ANCAGYRAVI EAAEHFGSFF TGQITAAGKV PPAKVLIIGA
     GVAGLAAIGA AVSLGAIVRA FDVRPEVKDQ VESLGAEFLQ VEMEGESGTG VGGYAKEMSE
     EFIKKEMEIF ARQAMEVDII ITTALIPGKP APKLITKGMV ESMKDGSVIV DLAAEQGGNC
     ELTEPGKVVK KFGATIIGYT DLPSRMPTTS SQLYATNLRH LLAELCPEKN GEINVNMDDV
     VIRGATVAKD GEITWPPPPI PVSKEEKPAP AAVAPPAAEE KKEEKKKLPK EWVTAIVMAI
     GGLALYGVGM VAPPEFLSHF TVFVLACFVG YMVIWNVTPA LHTPLMSVTN AISGIIVIGA
     LIQISSPGIL LKLLAGIAVL IASVNIFGGF WVTQRMLGMF RK
//
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