ID A0A150GRQ7_GONPE Unreviewed; 1182 AA.
AC A0A150GRQ7;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=Fanconi-associated nuclease {ECO:0000256|RuleBase:RU365033};
DE EC=3.1.4.1 {ECO:0000256|RuleBase:RU365033};
GN ORFNames=GPECTOR_9g569 {ECO:0000313|EMBL:KXZ52525.1};
OS Gonium pectorale (Green alga).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Volvocaceae; Gonium.
OX NCBI_TaxID=33097 {ECO:0000313|EMBL:KXZ52525.1, ECO:0000313|Proteomes:UP000075714};
RN [1] {ECO:0000313|Proteomes:UP000075714}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-2863 {ECO:0000313|Proteomes:UP000075714};
RX PubMed=27102219; DOI=10.1038/ncomms11370;
RA Hanschen E.R., Marriage T.N., Ferris P.J., Hamaji T., Toyoda A.,
RA Fujiyama A., Neme R., Noguchi H., Minakuchi Y., Suzuki M.,
RA Kawai-Toyooka H., Smith D.R., Sparks H., Anderson J., Bakaric R., Luria V.,
RA Karger A., Kirschner M.W., Durand P.M., Michod R.E., Nozaki H., Olson B.J.;
RT "The Gonium pectorale genome demonstrates co-option of cell cycle
RT regulation during the evolution of multicellularity.";
RL Nat. Commun. 7:11370-11370(2016).
CC -!- FUNCTION: Nuclease required for the repair of DNA interstrand cross-
CC links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of
CC DNA and cleaves DNA successively at every third nucleotide, allowing to
CC excise an ICL from one strand through flanking incisions.
CC {ECO:0000256|RuleBase:RU365033}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolytically removes 5'-nucleotides successively from the
CC 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.;
CC EC=3.1.4.1; Evidence={ECO:0000256|ARBA:ARBA00000983,
CC ECO:0000256|RuleBase:RU365033};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365033}.
CC -!- SIMILARITY: Belongs to the FAN1 family. {ECO:0000256|ARBA:ARBA00005533,
CC ECO:0000256|RuleBase:RU365033}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXZ52525.1}.
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DR EMBL; LSYV01000010; KXZ52525.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A150GRQ7; -.
DR STRING; 33097.A0A150GRQ7; -.
DR OrthoDB; 38749at2759; -.
DR Proteomes; UP000075714; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004528; F:phosphodiesterase I activity; IEA:UniProtKB-EC.
DR GO; GO:0036297; P:interstrand cross-link repair; IEA:InterPro.
DR CDD; cd22326; FAN1-like; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR033315; Fan1-like.
DR InterPro; IPR049132; FAN1-like_euk.
DR InterPro; IPR049126; FAN1-like_TPR.
DR InterPro; IPR049125; FAN1-like_WH.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR014883; VRR_NUC.
DR PANTHER; PTHR15749; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR PANTHER; PTHR15749:SF4; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR Pfam; PF21315; FAN1_HTH; 1.
DR Pfam; PF21170; FAN1_TPR; 1.
DR Pfam; PF08774; VRR_NUC; 1.
DR SMART; SM00990; VRR_NUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU365033};
KW DNA repair {ECO:0000256|RuleBase:RU365033};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU365033};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365033};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|RuleBase:RU365033};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365033};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU365033};
KW Nucleus {ECO:0000256|RuleBase:RU365033};
KW Reference proteome {ECO:0000313|Proteomes:UP000075714}.
FT DOMAIN 1051..1165
FT /note="VRR-NUC"
FT /evidence="ECO:0000259|SMART:SM00990"
FT REGION 226..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 454..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 530..584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 596..688
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 715..754
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 935..968
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 454..477
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 665..686
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1182 AA; 120153 MW; 3E2AB31BB6FDE76E CRC64;
MAEATTATAV KEAAVLMASV TGREGVIAAL AAAAAAAASA SLGGGATGGP AGPAAAAGEV
LRANLAVIAE DVRRHDGHLL SGEEAALLDR LLGLPPGPAC LFLRLALRKG PWFALAAIAY
TECGDAAEAA EVLLAAGLAE RPQPEDWPQV AALLPVAEVR SLAKGAPPLP PLPRAASAPS
ASAAVAPGRS SILSYTRRLT AAASVPIVAA ADGLAKAPQM VLTAPASPST TADALGRPSQ
TCSGVAAGDA KRVVKGADEG AGSAPRRQNG RAASGSGNAA AGGGNAAAAA GGKGVSGGRG
PGSGKAAVIE SLRQRHGDGS ACMSQKVAAR VGPCIRLAPA ATELLGRLQR LYFLGEGPAA
DMGRFLATDR GVIRYPRYTI RRSGSAFGSR QQLLEYEQAL QHAARLEECL EAGDEDGAAA
ALAPALAAVA SGAAQAVRWA GDATVPVRAV PGPWPWEDHP PAQPLPPLPP TAAARLPLPP
HPRDAKGGCD ELGPADGRAV CGAEGRQRGC GCSASAPAVA AVAAAAAVKG RTAARGTGAS
RRDSVDLTLS SDSDSGAGEE EGQEGEGSGP STGQDGHSHG GSGGFRGCCV GAARPGAADS
GVTGSTGDQE AGLPPDSDGV DGTGGYWAGL GVAPGAAGCR GSQMRESGSR APGGGGYPLE
AERAGFPPRR PDRCEPQRRD GGDRAADEGA TLYGADGCMP CDDGSHVYGD DDVVYGDELE
DGPGGAASPP GAACEDAPGA AQGRGAAGPG QGTVRDEAVL GGEEQPPLPQ QLHARLGQGQ
EGQPHGQSPA HPFLARFCAA WVYASMGTVG VSLLERQRRY PEAVALLRSL LRGRCCPGRR
GEWWTRMSLD LQHLGREEEA LQVAEAALTD PWVRHGDRLS LQRRVLRLGR PPRRWRRPAW
AEAALAEPRE VVVSATMLRG ATGFKSRFIV PHAAKARPPS DGDLPGGATA ADGPGAHGAG
GPDAAAGGDG TVGVEELALL HYRSPAGGGW RGVHSEGGVW GTLWGLLMWD VLFADVPEVF
RSPFQTAPLD LDTDAFLPSR RELVDARLQA IAGGAAPELL RGAWSAHEGT WARGVNWERQ
PLEELLEIAR CVGGLGLSVV CRLLCEDCAG WRGGMPDLLL WRPERGDAMV SEVKGPRDRL
SDQQRAWMAA LAAAEVRAEV LRVVEAAPGE AEGGGGGHSP LH
//
