ID A0A150H7M8_9MICO Unreviewed; 469 AA.
AC A0A150H7M8;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Ribosomal RNA small subunit methyltransferase B {ECO:0000313|EMBL:KXZ58102.1};
DE EC=2.1.1.176 {ECO:0000313|EMBL:KXZ58102.1};
GN Name=rsmB {ECO:0000313|EMBL:KXZ58102.1};
GN ORFNames=Bravens_01138 {ECO:0000313|EMBL:KXZ58102.1};
OS Brevibacterium ravenspurgense.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Brevibacteriaceae;
OC Brevibacterium.
OX NCBI_TaxID=479117 {ECO:0000313|EMBL:KXZ58102.1, ECO:0000313|Proteomes:UP000243589};
RN [1] {ECO:0000313|EMBL:KXZ58102.1, ECO:0000313|Proteomes:UP000243589}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG56047 {ECO:0000313|EMBL:KXZ58102.1,
RC ECO:0000313|Proteomes:UP000243589};
RA Bernier A.-M., Burdz T., Huynh C., Pachecho A.L., Wiebe D., Bonner C.,
RA Bernard K.;
RT "Use of Whole Genome Sequencing to ascertain that Brevibacterium
RT massiliense (Roux, Raoult 2009) is a later heterotypic synonym of
RT Brevibacterium ravenspurgense (Mages 2008).";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXZ58102.1}.
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DR EMBL; LQQC01000010; KXZ58102.1; -; Genomic_DNA.
DR RefSeq; WP_062021165.1; NZ_LQQC01000010.1.
DR AlphaFoldDB; A0A150H7M8; -.
DR PATRIC; fig|479117.4.peg.1135; -.
DR Proteomes; UP000243589; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.940.10; NusB-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR035926; NusB-like_sf.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF61; NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000243589};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 176..468
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 396
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 274..280
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 299
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 325
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 343
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 469 AA; 50404 MW; AEE1465936D98EDA CRC64;
MSRPDARRSR GRNNPQKANL ARILAWEVLV AVDEDDAYAN LLLPRKIART RLRADDAALA
TELTYGTLRA RGLYDAIIAE AASRKVDTLE IPVLNAMRMG AHQLLAMRIP DHAAVGETVA
VIKQSLPRAS GIVNAVLRRI GERSYDDWLA LMTAGLPEDQ AKSLRYSHPA WIVSALREAL
EAHGRSSAEI DLLLQSHNDP APVVLTALPG LDRSELPGTP TELSPLGVIL DRGDPGNLEA
VRTGRARVQD EGSQLTALTL LEAEAPEGEW LDMCAGPGGK TAILAATAAE RGVPVTALDA
SGHRADLVVD STRAFADTVE VFKGDGREFA EAYPGEFTRV LVDVPCSGLG ALRRRPEARW
RRSSEDVRTL RPLQDGLLRA AWQACAPGGV IAYSTCSPHK HETLDVVRAF AADTPAVQIL
NTPDHLARVT GQPAESFASS AVGDGAAAQL WSHVHETDGM FICLIRCPA
//
