ID A0A150J3C0_9EURY Unreviewed; 803 AA.
AC A0A150J3C0;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=CRISPR-associated nuclease/helicase Cas3 {ECO:0000313|EMBL:KYC51692.1};
DE EC=3.1.-.- {ECO:0000313|EMBL:KYC51692.1};
GN Name=cas3_1 {ECO:0000313|EMBL:KYC51692.1};
GN ORFNames=AMQ74_01028 {ECO:0000313|EMBL:KYC51692.1};
OS Candidatus Methanofastidiosum methylthiophilus.
OC Archaea; Euryarchaeota; Stenosarchaea group; Candidatus Methanofastidiosa;
OC Methanofastidiosum.
OX NCBI_TaxID=1705564 {ECO:0000313|EMBL:KYC51692.1, ECO:0000313|Proteomes:UP000075578};
RN [1] {ECO:0000313|EMBL:KYC51692.1, ECO:0000313|Proteomes:UP000075578}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=U1lsi0528_Bin089 {ECO:0000313|EMBL:KYC51692.1};
RX PubMed=26943620;
RA Nobu M.K., Narihiro T., Kuroda K., Mei R., Liu W.T.;
RT "Chasing the elusive Euryarchaeota class WSA2: genomes reveal a uniquely
RT fastidious methyl-reducing methanogen.";
RL ISME J. 0:0-0(2016).
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KYC51692.1}.
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DR EMBL; LNGD01000057; KYC51692.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A150J3C0; -.
DR PATRIC; fig|1705564.3.peg.1060; -.
DR Proteomes; UP000075578; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR CDD; cd17930; DEXHc_cas3; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR006935; Helicase/UvrB_N.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF04851; ResIII; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:KYC51692.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KYC51692.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 11..236
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 285..483
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 803 AA; 94890 MW; 2B5E88B2886462CC CRC64;
MEQKLFQFKL YSHPNKTLVS HLEEVGKKSK DILLQKNLNI GDYVDTEVLS EIIYLIGATH
DFGKASKYFQ EYLLEKDLTK KRELKSYKET RHGLFSSIFT YYIIKTYLKN IPKDKDEHYS
FLPIISFFVV KRHHGNLANS VKEINEIHEI LNDKKLISVI NSQIYSIDEN EIQNILDKLF
GCNKYLFSDF KENYLNILRE IDREFINLLK IQKNKILFYY FLTLLLYSIL IDSDKADAIG
SLNILRENIP SRLVDQYKIN KFNNNLNFID KIRTEIYEEV NSKLENLDVQ DKIYLLNVPT
GTGKTITSFS FAVKLKNKIQ TEKGYNPRII YSLPFLSIID QNYSVIEDII VSQFENIRSN
ILLKHHHLSE ISYTTSEEEY ESDKSLFLIE GWNSELIITS FIQLFHGLIS NKNRLLRKFH
NITNSIVILD EIQTIPHKYW KLLNESLYFL SKYFNTYFII VTATAPLIFD SEKNEINNLI
EDSQKYYSQL NRIKLNLYLE KEVNFEDFKN IVLNDIITRD DKSFLIVLNT ITSSKELFNF
IKKNTNNDCN EYYYLSSNII PFDRLDRIRK IKTSKKRKII VSTQVIEAGV DIDIDIIYRD
LAPLDSINQV AGRCNRNFSF KSQGEVNVYI LKDNNERKFC TYIYEGFLIN KTLETLKNVK
VVEEKDFFKL LNKYFLLVNE SSTDQISREI LENLYKLEFQ ETGNFKLIED DYEKIDIFIE
INENAKKIWN EFVKILDIDD PILKRKKFLN IKKSFYDYII SIPKNKANDV LYNERIGYVS
LGKINSKYDL ETGYKSEKIP LII
//
