ID   A0A150MBU8_9BACI        Unreviewed;       686 AA.
AC   A0A150MBU8;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   27-MAR-2024, entry version 29.
DE   RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE            EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN   ORFNames=B4135_1525 {ECO:0000313|EMBL:KYD22020.1};
OS   Caldibacillus debilis.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Caldibacillus.
OX   NCBI_TaxID=301148 {ECO:0000313|EMBL:KYD22020.1, ECO:0000313|Proteomes:UP000075683};
RN   [1] {ECO:0000313|EMBL:KYD22020.1, ECO:0000313|Proteomes:UP000075683}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B4135 {ECO:0000313|EMBL:KYD22020.1,
RC   ECO:0000313|Proteomes:UP000075683};
RA   Berendsen E.M., Wells-Bennik M.H., Krawcyk A.O., De Jong A., Holsappel S.,
RA   Eijlander R.T., Kuipers O.P.;
RT   "Draft Genome Sequences of Seven Thermophilic Sporeformers Isolated from
RT   Foods.";
RL   Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00034618};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KYD22020.1}.
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DR   EMBL; LQYT01000015; KYD22020.1; -; Genomic_DNA.
DR   RefSeq; WP_061568188.1; NZ_LQYT01000015.1.
DR   AlphaFoldDB; A0A150MBU8; -.
DR   STRING; 301148.B4135_1525; -.
DR   PATRIC; fig|301148.3.peg.1001; -.
DR   OrthoDB; 9810135at2; -.
DR   Proteomes; UP000075683; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   CDD; cd17932; DEXQc_UvrD; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR   InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR   InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014016; UvrD-like_ATP-bd.
DR   PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR   PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR   Pfam; PF00580; UvrD-helicase; 1.
DR   Pfam; PF13361; UvrD_C; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR   PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW   ProRule:PRU00560};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}; Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW   ProRule:PRU00560}.
FT   DOMAIN          1..334
FT                   /note="UvrD-like helicase ATP-binding"
FT                   /evidence="ECO:0000259|PROSITE:PS51198"
FT   DOMAIN          345..581
FT                   /note="UvrD-like helicase C-terminal"
FT                   /evidence="ECO:0000259|PROSITE:PS51217"
FT   BINDING         22..29
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ   SEQUENCE   686 AA;  80443 MW;  340C40229F4BDF03 CRC64;
     MNLTDEQKSI INSNERRLLV KAGAGTGKTE ILVRRIVRLI EENPDLSITD MAIITFTNKA
     TQEMQSRLKN AFYEKWKNEK FPKKKIKFRY ELESLNSAQI STIHKFCETI LQTIGPFQDE
     EIIYSPNFPV RSDQLTNTID HTVEEWIQWK KYTNKKIEHL NYMPVHQLKE IIRAAYKMVR
     SKGLDLDKLI ENTKRAAILE TNTTKKNLKI ELTELLKLVS ENHVKYKWDH LDVDDLLEYC
     AKILKKRPDL VQIIRKKYQY IFIDEFQDTS LYQAEIIKSI CDDSEGSPHL FVVGDIKQSI
     YEFRGANPES YQKVEHWIKQ TGKILTLSKN WRSTPEVVTY VNRVFGRIKA HEKYSFQNEP
     LEPSQEKERM KISDAYEWVF SENNESQGKI VANYLQKQIE EGKEENQFAV LVRTNYQLSI
     IADCLEQFGI RFEVADSGNF FNQTEIVHTN LVLKAILHEN PISANESLST IFFNNDVFLY
     NHVMSEIRTN SLSLRYTPSQ LLDYIYRKTD IFSRSSRQIK ANLNKLKELT RSLVKDENIT
     LGRYVNWLSA MIAANMDEPL ADVIEEDYCK VKLMTIHKAK GLEFPVVILP FLDYNISNEA
     LYPEVVVDPL HNCLEFSYDN YYYEGKGKIQ SDLYDEVIKK IQFHIYSEEL RVLYVALTRA
     KEKLVLIGNK NCRKDKICYQ NWLLQG
//