ID A0A150N0Y7_9BACI Unreviewed; 449 AA.
AC A0A150N0Y7;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Exodeoxyribonuclease 7 large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE EC=3.1.11.6 {ECO:0000256|HAMAP-Rule:MF_00378};
DE AltName: Full=Exodeoxyribonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
DE Short=Exonuclease VII large subunit {ECO:0000256|HAMAP-Rule:MF_00378};
GN Name=xseA {ECO:0000256|HAMAP-Rule:MF_00378};
GN ORFNames=B4113_0296 {ECO:0000313|EMBL:KYD30262.1};
OS Geobacillus sp. B4113_201601.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX NCBI_TaxID=1586290 {ECO:0000313|EMBL:KYD30262.1, ECO:0000313|Proteomes:UP000075654};
RN [1] {ECO:0000313|EMBL:KYD30262.1, ECO:0000313|Proteomes:UP000075654}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B4113_201601 {ECO:0000313|EMBL:KYD30262.1,
RC ECO:0000313|Proteomes:UP000075654};
RA Berendsen E.M., Wells-Bennik M.H., Krawcyk A.O., De Jong A., Holsappel S.,
RA Eijlander R.T., Kuipers O.P.;
RT "Draft Genome Sequences of Seven Thermophilic Sporeformers Isolated from
RT Foods.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Bidirectionally degrades single-stranded DNA into large acid-
CC insoluble oligonucleotides, which are then degraded further into small
CC acid-soluble oligonucleotides. {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in either 5'- to 3'- or 3'- to 5'-
CC direction to yield nucleoside 5'-phosphates.; EC=3.1.11.6;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355};
CC -!- SUBUNIT: Heterooligomer composed of large and small subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00378}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00378,
CC ECO:0000256|RuleBase:RU004355}.
CC -!- SIMILARITY: Belongs to the XseA family. {ECO:0000256|HAMAP-
CC Rule:MF_00378, ECO:0000256|RuleBase:RU004355}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KYD30262.1}.
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DR EMBL; LQYX01000014; KYD30262.1; -; Genomic_DNA.
DR RefSeq; WP_033019353.1; NZ_LQYX01000014.1.
DR AlphaFoldDB; A0A150N0Y7; -.
DR PATRIC; fig|1586290.3.peg.444; -.
DR OrthoDB; 9802795at2; -.
DR Proteomes; UP000075654; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0009318; C:exodeoxyribonuclease VII complex; IEA:InterPro.
DR GO; GO:0008855; F:exodeoxyribonuclease VII activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd04489; ExoVII_LU_OBF; 1.
DR HAMAP; MF_00378; Exonuc_7_L; 1.
DR InterPro; IPR003753; Exonuc_VII_L.
DR InterPro; IPR020579; Exonuc_VII_lsu_C.
DR InterPro; IPR025824; OB-fold_nuc-bd_dom.
DR NCBIfam; TIGR00237; xseA; 1.
DR PANTHER; PTHR30008; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR PANTHER; PTHR30008:SF0; EXODEOXYRIBONUCLEASE 7 LARGE SUBUNIT; 1.
DR Pfam; PF02601; Exonuc_VII_L; 1.
DR Pfam; PF13742; tRNA_anti_2; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00378};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_00378};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00378};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00378}.
FT DOMAIN 7..101
FT /note="OB-fold nucleic acid binding"
FT /evidence="ECO:0000259|Pfam:PF13742"
FT DOMAIN 124..439
FT /note="Exonuclease VII large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02601"
FT COILED 280..330
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 449 AA; 51178 MW; F8959B55AFA166BB CRC64;
MEVKYVTVGA LTKYIKRKFD VDPHLRDLWV KGEISNFKQH SRGHMYFTLK DSQGRIAAVM
FAGYNQHLPF RPEDGMNVLA RGEISVYEPN GNYQLYVKEM QPEGVGSLYL AYEQLKQRLE
AEGLFASAHK KPLPAFPRCI GVVTSPTGAA VRDIMTTIRR RYPLAKVILF PALVQGEGAA
SSIARAIERA NELGAVDVLI VGRGGGSIEE LWAFNEEMVA RAIFASRVPI ISAVGHETDF
TIADFVADLR APTPTAAAEL AVPHVGELAE RLAERKWRLI RAMKERLAAG RERLDRLQAS
YAFRYPKRLY EQKEQQFDAL LERLHRQRRS YVDEKHRQLR ELGLRLWRHH PAAELERVKE
RQIAAATALK KAMRSALERH AFRFRSHLAR LEALSPLRVM ERGYSLVYNE RGELVKRLSQ
LAVGERLSVR VQDGRVNCQV MGVEEKRDE
//
