ID A0A150UCW0_9BRAD Unreviewed; 687 AA.
AC A0A150UCW0;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU003887};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU003887};
GN ORFNames=SE92_18810 {ECO:0000313|EMBL:KYG22036.1};
OS Bradyrhizobium sp. AT1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=574934 {ECO:0000313|EMBL:KYG22036.1, ECO:0000313|Proteomes:UP000075342};
RN [1] {ECO:0000313|EMBL:KYG22036.1, ECO:0000313|Proteomes:UP000075342}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AT1 {ECO:0000313|EMBL:KYG22036.1,
RC ECO:0000313|Proteomes:UP000075342};
RA Okubo T., Minamisawa K.;
RT "Evolution of nitrogen fixation genes.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|ARBA:ARBA00000073};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RsuA family.
CC {ECO:0000256|ARBA:ARBA00008348, ECO:0000256|RuleBase:RU003887}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KYG22036.1}.
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DR EMBL; JXDL01000001; KYG22036.1; -; Genomic_DNA.
DR RefSeq; WP_063197333.1; NZ_JXDL01000001.1.
DR AlphaFoldDB; A0A150UCW0; -.
DR STRING; 574934.SE92_18810; -.
DR PATRIC; fig|574934.3.peg.3989; -.
DR Proteomes; UP000075342; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.70.1560; Alpha-L RNA-binding motif; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR042092; PsdUridine_s_RsuA/RluB/E/F_cat.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR000748; PsdUridine_synth_RsuA/RluB/E/F.
DR InterPro; IPR018496; PsdUridine_synth_RsuA/RluB_CS.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00093; pseudouridine synthase; 1.
DR PANTHER; PTHR47683; PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47683:SF3; RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01149; PSI_RSU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003887};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 369..428
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT REGION 1..369
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 615..687
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..369
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 620..664
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 687 AA; 78796 MW; BE91DED5448F8CCC CRC64;
MPRDSDKDND SRGRRGPARG PSKGGRSGKA RGPEKKFAKR GFEGKSEGDK RPPRGDRDSR
PPRADRGDRP FRRREEGDAP RRDFSDRPKF KRDDRGSEGR GERSFKPRGD RPFSDSSSRD
GEKRPFKPRG DRPSFGRDDR PPRRDRDDAR PAGRSSDRKF GDKKPYAPRG DRPERKFDGE
RKFSRGGPRE DRGERSFKPR GDRPNFDRGD RPPRGDRPER KFDGERKFSR GAPDRDRGDR
GPRKDFGGRD RGADKPWQKR DDRREGGRGD DRPRFSRARD DRPSGDRPFR DRPKFDRPRE
RPEGRSDWHE HPRNEGRFGD RPRRENEDES RIFEKRPAFG GRGAYRERDR DSDRRPRREE
EPKPKKAGER IAKALARAGL ASRRDAEEMV TQGRVTVNGR VINSPALDIT KNDVVLVDGK
PLPERERTRL FLYHKPRGLM TTHDDPEGRP TVFDNLPEGL PRLISVGRLD FNTEGLLLLT
NDGGLARTLE LPDTGWLRRY RVRAHGDVTQ AQLDALKDGV EIEGVKYGPI DATLERDQGA
NVWLVFAIRE GKNREVRNVC AHLGLEVNRL IRVSYGPFQL GEVPEGQVEE IKSRVLREQL
GDKIIEKSGA QFDVPSKASR VEDAPSEKKP ASKRGVIADR KGRRVLVQRT GSEEARERNE
EQASGYGPPR RPKRGYHGKR DLTPRED
//
