ID A0A151ARN5_9CLOT Unreviewed; 527 AA.
AC A0A151ARN5;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=1,4-alpha-glucan branching enzyme {ECO:0000313|EMBL:KYH30243.1};
DE EC=2.4.1.18 {ECO:0000313|EMBL:KYH30243.1};
GN ORFNames=CLCOL_01870 {ECO:0000313|EMBL:KYH30243.1};
OS Clostridium colicanis DSM 13634.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1121305 {ECO:0000313|EMBL:KYH30243.1, ECO:0000313|Proteomes:UP000075374};
RN [1] {ECO:0000313|EMBL:KYH30243.1, ECO:0000313|Proteomes:UP000075374}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 13634 {ECO:0000313|EMBL:KYH30243.1,
RC ECO:0000313|Proteomes:UP000075374};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium colicanis DSM 13634.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 57 family.
CC {ECO:0000256|ARBA:ARBA00006821, ECO:0000256|RuleBase:RU361196}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KYH30243.1}.
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DR EMBL; LTBB01000001; KYH30243.1; -; Genomic_DNA.
DR RefSeq; WP_061857130.1; NZ_LTBB01000001.1.
DR AlphaFoldDB; A0A151ARN5; -.
DR STRING; 1121305.CLCOL_01870; -.
DR PATRIC; fig|1121305.3.peg.185; -.
DR Proteomes; UP000075374; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0030979; P:alpha-glucan biosynthetic process; IEA:InterPro.
DR CDD; cd10792; GH57N_AmyC_like; 1.
DR Gene3D; 1.20.1430.10; Families 57/38 glycoside transferase, middle domain; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR InterPro; IPR037090; 57_glycoside_trans_central.
DR InterPro; IPR015293; BE_C.
DR InterPro; IPR040042; Branching_enz_MT3115-like.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR004300; Glyco_hydro_57_N.
DR PANTHER; PTHR41695; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR PANTHER; PTHR41695:SF1; 1,4-ALPHA-GLUCAN BRANCHING ENZYME RV3031-RELATED; 1.
DR Pfam; PF09210; BE_C; 1.
DR Pfam; PF03065; Glyco_hydro_57; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|RuleBase:RU361196};
KW Glycosyltransferase {ECO:0000313|EMBL:KYH30243.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000075374};
KW Transferase {ECO:0000313|EMBL:KYH30243.1}.
FT DOMAIN 8..324
FT /note="Glycoside hydrolase family 57 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03065"
FT DOMAIN 425..524
FT /note="1,4-alpha-glucan branching enzyme C-terminal"
FT /evidence="ECO:0000259|Pfam:PF09210"
FT ACT_SITE 189
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT ACT_SITE 350
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-1"
FT BINDING 241
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 258
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 404
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
FT BINDING 464
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR640042-2"
SQ SEQUENCE 527 AA; 62348 MW; B99113A3719D0FE3 CRC64;
MTKGYVSLVL HSHMPFIRHK EVNDALEERW LFEAMSECYI PLIEVYDGLI KDNIKFKITM
SITPPLMSML EDEYLNNRYL QYLKKSIELS EKEIVRTKED KELQKLAIFY NNRFKKILSI
YKSYDYRLMN AFKKFDKLGC LEIITCSATH ALLPLISINP EAVRAQIATA VKSYTDCIGH
EPKGIWLPEC AYDYFLDDIL KEYGIEYFIS ESKAVLHAEP RPRYGTYAPI ATSNGICTFG
RDMESSRQVW SSFMGYPGDF NYREFYRDLG YDAPMEYIAP YINKSGIRVD TGIKYYKITG
KTEFKEYYNR ENAIKKVKEH AAHFAEERNY QIVSLHQDMG VKPIIVCPYD TELYGHWWFE
GPDFINEFIR KSSEDWIYYE LTTPGEYLEK YPVVQCCRPC PSSWGENSDY SVWLNPSNHW
VYRDLHKCEQ KMIRLSNTYS NPRELEERAL NQAARELMLA EASDWPFIIK NNTTVEYAVK
RINTHVDRFN KLYEQITKDS IDERWLSSIE ELDNIFPNMD YRIYMSR
//
