ID A0A151U0W5_CAJCA Unreviewed; 461 AA.
AC A0A151U0W5;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=tRNA pseudouridine synthase {ECO:0000313|EMBL:KYP72956.1};
GN ORFNames=KK1_005561 {ECO:0000313|EMBL:KYP72956.1};
OS Cajanus cajan (Pigeon pea) (Cajanus indicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Cajanus.
OX NCBI_TaxID=3821 {ECO:0000313|EMBL:KYP72956.1, ECO:0000313|Proteomes:UP000075243};
RN [1] {ECO:0000313|EMBL:KYP72956.1, ECO:0000313|Proteomes:UP000075243}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Asha {ECO:0000313|Proteomes:UP000075243};
RX PubMed=22057054; DOI=10.1038/nbt.2022;
RA Varshney R.K., Chen W., Li Y., Bharti A.K., Saxena R.K., Schlueter J.A.,
RA Donoghue M.T., Azam S., Fan G., Whaley A.M., Farmer A.D., Sheridan J.,
RA Iwata A., Tuteja R., Penmetsa R.V., Wu W., Upadhyaya H.D., Yang S.P.,
RA Shah T., Saxena K.B., Michael T., McCombie W.R., Yang B., Zhang G.,
RA Yang H., Wang J., Spillane C., Cook D.R., May G.D., Xu X., Jackson S.A.;
RT "Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop
RT of resource-poor farmers.";
RL Nat. Biotechnol. 30:83-89(2012).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; CM003604; KYP72956.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A151U0W5; -.
DR STRING; 3821.A0A151U0W5; -.
DR OMA; IEISIWG; -.
DR Proteomes; UP000075243; Chromosome 2.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 2.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF9; TRNA PSEUDOURIDYLATE SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 2.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000075243};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 273..352
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 175..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 59
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 124
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 461 AA; 52480 MW; 7E1C0063E0CF61F6 CRC64;
MRVAYVGTNY RGLQMQTDQH SISTIEKELE TAIFKAGGIR ESNFGDLQKI RWARSSRTDK
GVHSLATIIS FKMEIPENAW NGDPYGFSLA NYVNSHLPWD IKVLSILPSQ RSFGPRKECV
FRKYSYLLPA DIIGIKSHSS NDEIDYHISE FNDILNVFEG EHPFHNYTAR SKYRKNFPHR
QSSPKSDKSE SESLLPYASD CEYSDGEENS EIDEAITENI ACQSKNCSET RHFGEPVVGS
SNKNGNGLLD QDSKLVARAR WLYEPDETDR LSASHFRKVF QCSCGKLKTS LGYNYIEISI
EGESFMLHQI RKMVGTAVAV KRKLLPKDII KLSLARFSRI ILPLAPSEVL ILSGNRFSIR
TRPGNVTRPE MRTIVESEEI NKAVDEFYSS VMLPQVSKFL DPSRSPWEEW IEKLDAHTSI
PNDQLDDVRK AWNLLKENLE NTIKHSSVVS EGKKVTIALL F
//
