GenomeNet

Database: UniProt
Entry: A0A151U8P3_CAJCA
LinkDB: A0A151U8P3_CAJCA
Original site: A0A151U8P3_CAJCA 
ID   A0A151U8P3_CAJCA        Unreviewed;       805 AA.
AC   A0A151U8P3;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   31-JUL-2019, entry version 19.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   ORFNames=KK1_019891 {ECO:0000313|EMBL:KYP75695.1};
OS   Cajanus cajan (Pigeon pea) (Cajanus indicus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae;
OC   Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;
OC   50 kb inversion clade; NPAAA clade; indigoferoid/millettioid clade;
OC   Phaseoleae; Cajanus.
OX   NCBI_TaxID=3821 {ECO:0000313|EMBL:KYP75695.1, ECO:0000313|Proteomes:UP000075243};
RN   [1] {ECO:0000313|EMBL:KYP75695.1, ECO:0000313|Proteomes:UP000075243}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Asha {ECO:0000313|Proteomes:UP000075243};
RX   PubMed=22057054; DOI=10.1038/nbt.2022;
RA   Varshney R.K., Chen W., Li Y., Bharti A.K., Saxena R.K.,
RA   Schlueter J.A., Donoghue M.T., Azam S., Fan G., Whaley A.M.,
RA   Farmer A.D., Sheridan J., Iwata A., Tuteja R., Penmetsa R.V., Wu W.,
RA   Upadhyaya H.D., Yang S.P., Shah T., Saxena K.B., Michael T.,
RA   McCombie W.R., Yang B., Zhang G., Yang H., Wang J., Spillane C.,
RA   Cook D.R., May G.D., Xu X., Jackson S.A.;
RT   "Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume
RT   crop of resource-poor farmers.";
RL   Nat. Biotechnol. 30:83-89(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   -----------------------------------------------------------------------
DR   EMBL; CM003603; KYP75695.1; -; Genomic_DNA.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000075243; Chromosome 1.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR040881; Urease_linker.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 2.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   Pfam; PF18473; Urease_linker; 1.
DR   PIRSF; PIRSF001222; Urease; 2.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000075243};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700, ECO:0000313|EMBL:KYP75695.1};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222};
KW   Reference proteome {ECO:0000313|Proteomes:UP000075243}.
FT   DOMAIN      400    805       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    562    562       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   BINDING     490    490       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     488    488       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   805 AA;  86516 MW;  8B5DB61956EA59A3 CRC64;
     MKLSPREIEK LSLHNAGYLA QKRLARGLRL NYAETVALIA TQIVEFVRDG DKTVAQLMSI
     GRELLGRRQV LPAVPHLVES VQVEATFRDG TELVTIHDPI ACENGNLELA LFGSFLPVPS
     LDKFTANDED NRIPGEIIVV GGNLVLNHGR KAVILKVVNN GDRPVQVGSH YHFIELNPYL
     TFDRRKAYGM RLNIAAGNAT RFEPGESKSV LLVSIGGNKV IRGGNNIADG PVNDSNCIAA
     MQAVITRGFG HVEEENAREG VTGEDYSLTT VISREEYANK YGPTTGDKIR LGDTNLFAEI
     EKDFAIYGDE CIFGGGKVIR DGMGQSCGHP PEGSLDTVIT NAVIIDYSGI IKADIGIKDG
     LIISIGKSGN PDVMDGVFHD MIIGANTEVI AGEGLIVTAG AIDCHVHFIC PQLVDDAVTS
     GITTLVGGGT GPAEGTRATT CTPAPIQMKF MLQSTDDMPL NFGFTGKGNC AKPDELHEII
     KAGAMGLKLH EDWGTTPAAI DSCLTIAEQY DIQAQLCAGG GHAPDIIKVC GEKNVLPSST
     NPTRPYTSNT IDEHVDMLMV CHHLKRNIPE DVAFSESRIR AETIAAEDIL HDMGAISIIS
     SDSQAMGRIG EVISRTWQTA DKMKSQRGPL QPGEENDNLR IKRYVAKYTI NPAIANGFSQ
     YIGSVEVGKL ADLVLWKPSF FGAKPEMVIK GGEVAYANMG DPNASIPTPE PVIMRPMFGA
     FGKAGSAHSI AFAALDNGVK ASYGLNKRVE AVENVRKLTK LDMKLNDALP EITVEPESYT
     VTADGVVLTC AAATTVPLSR NYFIF
//
DBGET integrated database retrieval system