ID A0A158HHA5_9BURK Unreviewed; 413 AA.
AC A0A158HHA5;
DT 02-NOV-2016, integrated into UniProtKB/TrEMBL.
DT 02-NOV-2016, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=carboxymethylenebutenolidase {ECO:0000256|ARBA:ARBA00044062};
DE EC=3.1.1.45 {ECO:0000256|ARBA:ARBA00044062};
GN ORFNames=AWB69_04396 {ECO:0000313|EMBL:SAL43419.1};
OS Caballeronia udeis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Caballeronia.
OX NCBI_TaxID=1232866 {ECO:0000313|EMBL:SAL43419.1, ECO:0000313|Proteomes:UP000054683};
RN [1] {ECO:0000313|EMBL:SAL43419.1, ECO:0000313|Proteomes:UP000054683}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LMG 27134 {ECO:0000313|EMBL:SAL43419.1};
RA Oliw E.H.;
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC Evidence={ECO:0000256|ARBA:ARBA00043789};
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000256|ARBA:ARBA00008456}.
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DR EMBL; FCOK02000030; SAL43419.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A158HHA5; -.
DR Proteomes; UP000054683; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.10.450.50; -; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR PANTHER; PTHR46623; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR PANTHER; PTHR46623:SF11; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR Pfam; PF01738; DLH; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR SUPFAM; SSF54427; NTF2-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:SAL43419.1}.
FT DOMAIN 17..231
FT /note="Dienelactone hydrolase"
FT /evidence="ECO:0000259|Pfam:PF01738"
SQ SEQUENCE 413 AA; 45645 MW; 1FA966A098219C90 CRC64;
MMSGQTIQVT AYDGGKFAAY LAKPAQGSGP GLVLLQEIFG INGYMKSMAD RYAEEGYVVL
VPDLFWRMKP GVELGYGEAD FKEALGYNEK LDEENAVNDI AATIAAIRAL PEQAGKVGTI
GYCLGGRLAM LAAARTDVDC AVSYYGVGLD AYTSKIPAIH CPMIFHFAEN DAMTPTESRE
KIIAALKQKP SIETYVYPGC DHAFATPERE HFDKPAAMMA YSRTMALLRK VLGPVYDLNQ
LWEMHCYYEF GTRDVNAVMP TMVAQPYVNH VPTMTGGVGH DQLKRFYKYH FVDSNPADTR
LIPVSRTIGA DRVVDEFVFC ATHDREIDWL LPGLKPTGKY FEVPMLAVIC FRGDKLYNEH
IYWDQASVLV QIGALDPTGL PVAGIQTAKK MVDETLPTNT LMPNWASSEG KPV
//
