ID A0A161I1A3_9MICO Unreviewed; 1181 AA.
AC A0A161I1A3;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN Name=pcrA {ECO:0000313|EMBL:ANC30985.1};
GN ORFNames=I598_1429 {ECO:0000313|EMBL:ANC30985.1};
OS Isoptericola dokdonensis DS-3.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales;
OC Promicromonosporaceae; Isoptericola.
OX NCBI_TaxID=1300344 {ECO:0000313|EMBL:ANC30985.1, ECO:0000313|Proteomes:UP000076794};
RN [1] {ECO:0000313|EMBL:ANC30985.1, ECO:0000313|Proteomes:UP000076794}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS-3 {ECO:0000313|EMBL:ANC30985.1,
RC ECO:0000313|Proteomes:UP000076794};
RA Kwon S.-K., Kim J.F.;
RT "Complete genome sequence of a soil Actinobacterium, Isoptericola
RT dokdonensis DS-3.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP014209; ANC30985.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A161I1A3; -.
DR STRING; 1300344.I598_1429; -.
DR KEGG; ido:I598_1429; -.
DR PATRIC; fig|1300344.3.peg.1433; -.
DR OrthoDB; 4812256at2; -.
DR Proteomes; UP000076794; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF55; DNA HELICASE; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000076794}.
FT DOMAIN 65..395
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 400..744
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 552..577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 873..921
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 552..567
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 86..93
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1181 AA; 125438 MW; 7033861BC3788D67 CRC64;
MTSHPTGDQL PEDDGHATLG DETWWAALDA DHRDTDLGFP ELESPVDVSR PRYSARDIAE
KLGRPAPTDE QVAVIEAPLE PVLVVAGAGS GKTETMAARV VWLIANGLVE PEEVLGLTFT
RKAAGELSTR VQSRLAQLAR AEGRTTADLD LLARPTVATY NAYAASLVAD HGLRLGVEPG
SRLLGEANQW QLAAEVVESW DGDLGTDKAV STVVGAVLSL SGALGEHLRG VDEARDVLDG
IVERLDSLPL GPRQRARTQD VEKLIRSVAE RARLLDLVAE YRRRKRVADS LDFGDQVAFA
AELARTVPAV GAAERTRFRA VLLDEYQDTS FAQVELLAGL FGGGHAVTAV GDPHQSIYGW
RGASAAGLSR FPDRFRHADG GAAPVRFLST SWRNDAAVLA AANVASGPLR DAGTAVPVPP
LHLRDGAGQG VVRAHVAATL EEEAEVVAEL VAARWRRGTR PDGADRVTAA VLCRARSQFA
VLETALRRRG LPVEVVGLGG LLTTPEVVDV VALLEAVHDP SRGDSLLRLL TGPRVNLGAA
DLHALGSSAA DLARRDDPRR RHAAAGAPDD APGDVPDGDE VTVVEGDVVD HRSLVDALDD
LPEPGRAARD GRVLTPEGRT RLAALARLLR DLRSLTYLSL PELVVAAERA LGLDVEVATA
ELLADGLAGA GPYAADGVSR RGREHLDAFR DVAASFAQSA DVATLGAFLA WLGVADDQER
GLDMPVREPD PDAVQIITAH AAKGLEWDVV AVPGLVDGVF PTVAESSRGR TSPGWLSGLG
ELPYPLRGDA EDLPGLDLAS VADTKELMAR RDEFVLDCGA HQLAEERRLA YVAFTRARRE
LFCTAHWWGT AAKPRALSPF LQELAEAGLV STEGWEPAPD GTDNPRDGEV PTGVWPAPEA
GGTAPEPAVP GSAPTAPGDV PTARDLLRRT AARVRAAADA EPADGGLVDE TGHDLVGLAR
LLLAERADRV EPSVEMPAHV SASGLVRLAR DRDEFARQLR RPVPVEPTVH ARRGTLFHAW
VEQYFGAASL LDVEDMPGAE DAGDHSGDLA LDELKELFLG TPWAALTPVA VEQDVETPVG
GVMVRSRIDA VFPDPDAPEP APGEAPAVVV VDWKTGRAPR DPVDRAQREV QLAAYRLAWS
RWKGIPVEKV SAAFVHVADG TTVRPTELLD EAGLEALVRG A
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