ID   A0A161WQN4_DAUCS        Unreviewed;       432 AA.
AC   A0A161WQN4;
DT   06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT   06-JUL-2016, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=mannan endo-1,4-beta-mannosidase {ECO:0000256|ARBA:ARBA00012706};
DE            EC=3.2.1.78 {ECO:0000256|ARBA:ARBA00012706};
GN   ORFNames=DCAR_009749 {ECO:0000313|EMBL:KZN00995.1};
OS   Daucus carota subsp. sativus (Carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=79200 {ECO:0000313|EMBL:KZN00995.1};
RN   [1] {ECO:0000313|EMBL:KZN00995.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Leaf {ECO:0000313|EMBL:KZN00995.1};
RX   PubMed=27158781; DOI=10.1038/ng.3565;
RA   Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA   Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA   Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA   Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT   "A high-quality carrot genome assembly provides new insights into
RT   carotenoid accumulation and asterid genome evolution.";
RL   Nat. Genet. 48:657-666(2016).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random hydrolysis of (1->4)-beta-D-mannosidic linkages in
CC         mannans, galactomannans and glucomannans.; EC=3.2.1.78;
CC         Evidence={ECO:0000256|ARBA:ARBA00001678};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000256|ARBA:ARBA00005641, ECO:0000256|RuleBase:RU361153}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KZN00995.1}.
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DR   EMBL; LNRQ01000003; KZN00995.1; -; Genomic_DNA.
DR   RefSeq; XP_017242595.1; XM_017387106.1.
DR   RefSeq; XP_017242596.1; XM_017387107.1.
DR   AlphaFoldDB; A0A161WQN4; -.
DR   STRING; 79200.A0A161WQN4; -.
DR   EnsemblPlants; KZN00995; KZN00995; DCAR_009749.
DR   GeneID; 108214871; -.
DR   Gramene; KZN00995; KZN00995; DCAR_009749.
DR   KEGG; dcr:108214871; -.
DR   OMA; TNNSWGN; -.
DR   OrthoDB; 2717493at2759; -.
DR   Proteomes; UP000077755; Chromosome 3.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016985; F:mannan endo-1,4-beta-mannosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0071704; P:organic substance metabolic process; IEA:InterPro.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR045053; MAN-like.
DR   PANTHER; PTHR31451:SF45; MANNAN ENDO-1,4-BETA-MANNOSIDASE 2; 1.
DR   PANTHER; PTHR31451; MANNAN ENDO-1,4-BETA-MANNOSIDASE 7; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361153};
KW   Hydrolase {ECO:0000256|RuleBase:RU361153};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000077755};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        7..25
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          75..353
FT                   /note="Glycoside hydrolase family 5"
FT                   /evidence="ECO:0000259|Pfam:PF00150"
SQ   SEQUENCE   432 AA;  49758 MW;  B25B7C9AEEDFED39 CRC64;
     MVAGNNLFYP IVGFASCVAF LYMSFGDLWA DFGDHSEQIG SFVERNGTHF MVDGRVFYVN
     GWNSYWLMDD SVNEYSKYRV KAMLQAGAKM GLTVCRTWAF NDGDYNALQI SPGQFDEKVF
     RALDHVIAEA KQQGVRLLLA LVNNLQAYGG KTQYVKWAWE EGLGLTSSND SFFYDPSIRR
     YFKHYLKTIL TRKNSLTGIE YRDDPTIFGW ELINEPRCVT DASGNTLQDW IQEMSTFLKA
     IDRKHLLTVG LEGFYGPKSP KRLTENPEWW ASDLGTDFIR NSMLPTIDFA SVHIYPDHWT
     HVQDLEYKLK FVSTWMQSHI EDGDKELMKP IMFTEFGLSS ENKGFDPAQR DRFYKIVFDI
     IYKSAKEKGS GAGSFAWQFF VGGMEDHNDE FGIVPWQRPS TYKLITEQSC RLARIQGVIP
     LQTQYLKQLC SH
//