ID   A0A161ZR96_DAUCS        Unreviewed;       663 AA.
AC   A0A161ZR96;
DT   06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT   06-JUL-2016, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE            EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN   ORFNames=DCAR_026125 {ECO:0000313|EMBL:KZM89050.1};
OS   Daucus carota subsp. sativus (Carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=79200 {ECO:0000313|EMBL:KZM89050.1};
RN   [1] {ECO:0000313|EMBL:KZM89050.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   TISSUE=Leaf {ECO:0000313|EMBL:KZM89050.1};
RX   PubMed=27158781; DOI=10.1038/ng.3565;
RA   Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA   Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA   Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA   Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT   "A high-quality carrot genome assembly provides new insights into
RT   carotenoid accumulation and asterid genome evolution.";
RL   Nat. Genet. 48:657-666(2016).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KZM89050.1}.
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DR   EMBL; LNRQ01000007; KZM89050.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A161ZR96; -.
DR   EnsemblPlants; KZM89050; KZM89050; DCAR_026125.
DR   Gramene; KZM89050; KZM89050; DCAR_026125.
DR   OMA; KEGTTCG; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000077755; Chromosome 7.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd16664; RING-Ubox_PUB; 1.
DR   Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR   Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000225; Armadillo.
DR   InterPro; IPR045210; RING-Ubox_PUB.
DR   InterPro; IPR003613; Ubox_domain.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   PANTHER; PTHR23315:SF116; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR   PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR   Pfam; PF00514; Arm; 1.
DR   Pfam; PF04564; U-box; 1.
DR   SMART; SM00185; ARM; 3.
DR   SMART; SM00504; Ubox; 1.
DR   SUPFAM; SSF48371; ARM repeat; 1.
DR   SUPFAM; SSF57850; RING/U-box; 1.
DR   PROSITE; PS50176; ARM_REPEAT; 2.
DR   PROSITE; PS51698; U_BOX; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000077755};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT   DOMAIN          277..351
FT                   /note="U-box"
FT                   /evidence="ECO:0000259|PROSITE:PS51698"
FT   REPEAT          421..463
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT   REPEAT          546..589
FT                   /note="ARM"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
SQ   SEQUENCE   663 AA;  74072 MW;  C7BFEC372712025B CRC64;
     MINNFDQNDR RILTFPAIHP CENIYPGTLL ESLVTLSRKI SNYQTRFFAT QRKNSKQAIR
     QVGILLMFLE ELQESRIVLP GSINLCFSEL HLTFQRILFL LDDITREGAR IWVLMKAHFV
     ASQFLVLVRS VATALDVLEM DSVRVSSEIR ELVELVAIQA RRVRLELDFE DEEVMKKVIV
     IINQFENRFE PDQGMIKGVL DYLGIRRWDE CHKEVKFLEE LSGLMCLDGN EREMPLISSL
     AGLMRYCRGV IFTNSGFGEG GQVDGRGNVE VEVVSFLNLE DFRCPISLEL MTDPVTVSTG
     QTYDRASIQK WFKSGNLICP KTGERLETTE LVANSSLRKI IQQFCADHGV SLAKTRKKNS
     DISRTIVPGS PAAAEAIRFL SGFLSSRLSY GTDEQKMKAA HEVRLLGKSS IFNRYCLIEA
     GTVPPLLTLL ASSDSPTQEN AISAILKLSK HSKGKKQIIE NEGLNLIVNV LKKGLKLEAR
     QTAAATIFYL SSVDKYRKML GETTEAIPAL VELIKEGTNC GKKNAVVAIF GLLLYPRNHH
     RVLAAGTVQV LTNLLSTSDK KDVITDSLAI LAKLSESFEG SSMIVQASAL SIILRIFKNL
     ASRVLAKDPG LMTALYSLLS DVDGTAHSNK KARMIINIVH RFHETSTSQL INHQVQEQSI
     HVQ
//