ID A0A162AGV6_DAUCS Unreviewed; 304 AA.
AC A0A162AGV6;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Translocase of chloroplast {ECO:0000256|PIRNR:PIRNR038134};
DE EC=3.6.5.- {ECO:0000256|PIRNR:PIRNR038134};
GN ORFNames=DCAR_009465 {ECO:0000313|EMBL:KZN00711.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZN00711.1};
RN [1] {ECO:0000313|EMBL:KZN00711.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaf {ECO:0000313|EMBL:KZN00711.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- FUNCTION: GTPase involved in protein precursor import into
CC chloroplasts. Seems to recognize chloroplast-destined precursor
CC proteins and regulate their presentation to the translocation channel
CC through GTP hydrolysis. {ECO:0000256|PIRNR:PIRNR038134}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|PIRNR:PIRNR038134}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast outer membrane
CC {ECO:0000256|PIRNR:PIRNR038134}.
CC -!- SIMILARITY: Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like
CC GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family.
CC TOC34 subfamily. {ECO:0000256|PIRNR:PIRNR038134}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZN00711.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LNRQ01000003; KZN00711.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A162AGV6; -.
DR STRING; 79200.A0A162AGV6; -.
DR EnsemblPlants; KZN00711; KZN00711; DCAR_009465.
DR Gramene; KZN00711; KZN00711; DCAR_009465.
DR OMA; QRGKLWI; -.
DR Proteomes; UP000077755; Chromosome 3.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblPlants.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0015450; F:protein-transporting ATPase activity; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IEA:UniProtKB-UniRule.
DR CDD; cd01853; Toc34_like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR006703; G_AIG1.
DR InterPro; IPR045058; GIMA/IAN/Toc.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005688; Toc34.
DR NCBIfam; TIGR00991; 3a0901s02IAP34; 1.
DR PANTHER; PTHR10903; GTPASE, IMAP FAMILY MEMBER-RELATED; 1.
DR PANTHER; PTHR10903:SF149; TRANSLOCASE OF CHLOROPLAST 33, CHLOROPLASTIC; 1.
DR Pfam; PF04548; AIG1; 1.
DR PIRSF; PIRSF038134; Toc34; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51720; G_AIG1; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|PIRNR:PIRNR038134};
KW GTP-binding {ECO:0000256|PIRNR:PIRNR038134};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR038134};
KW Magnesium {ECO:0000256|PIRSR:PIRSR038134-2};
KW Membrane {ECO:0000256|PIRNR:PIRNR038134};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR038134-2};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR038134}; Plastid {ECO:0000256|PIRNR:PIRNR038134};
KW Plastid outer membrane {ECO:0000256|PIRNR:PIRNR038134};
KW Protein transport {ECO:0000256|PIRNR:PIRNR038134};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755};
KW Transmembrane {ECO:0000256|PIRNR:PIRNR038134};
KW Transport {ECO:0000256|PIRNR:PIRNR038134}.
FT DOMAIN 35..262
FT /note="AIG1-type G"
FT /evidence="ECO:0000259|PROSITE:PS51720"
FT BINDING 47..52
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-1"
FT BINDING 51
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-2"
FT BINDING 66..71
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-1"
FT BINDING 69
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-2"
FT BINDING 161
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-1"
FT BINDING 206..207
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|PIRSR:PIRSR038134-1"
SQ SEQUENCE 304 AA; 33589 MW; 73997AD647EF8393 CRC64;
MASPLIREWT GIQQFPPATQ TTLIELLAQL KEKDVSELTI LVMGKGGVGK SSTVNSIIGE
RAVTVSAFQS ENPRPVMVSR LRSGFTFNII DTPGLVEGGY VNDQALEMIK RFLLNKTIDV
LLYVDRLDAY RVDNLDRQIV NAITDSFGKD IWRRGIVVLT HAQLSPPDGL GYDEFFSRRS
EALLKVVGLG AKIKKIQDAI PVVLVENSGR CTKNDSDEKV GLLSNILPNG VAWIPNVVKT
ITEVVSNGSK SIFVDKKLID GPNPNEKGKW FIPLIAAFQY FFIVGHYQNL IKKDIAAEKE
SSKS
//
