ID A0A162H4R9_9GAMM Unreviewed; 490 AA.
AC A0A162H4R9;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN ORFNames=A3709_16275 {ECO:0000313|EMBL:KZX59107.1};
OS Halioglobus sp. HI00S01.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Cellvibrionales; Halieaceae;
OC Halioglobus.
OX NCBI_TaxID=1822214 {ECO:0000313|EMBL:KZX59107.1, ECO:0000313|Proteomes:UP000077184};
RN [1] {ECO:0000313|EMBL:KZX59107.1, ECO:0000313|Proteomes:UP000077184}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HI00S01 {ECO:0000313|EMBL:KZX59107.1,
RC ECO:0000313|Proteomes:UP000077184};
RA Sosa O.A.;
RT "Microbial cycling of marine high molecular weight dissolved organic
RT matter.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZX59107.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LWEE01000025; KZX59107.1; -; Genomic_DNA.
DR RefSeq; WP_066051514.1; NZ_LWEE01000025.1.
DR AlphaFoldDB; A0A162H4R9; -.
DR OrthoDB; 9810950at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000077184; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR014292; Acyl_transf_WS/DGAT.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR NCBIfam; TIGR02946; acyl_WS_DGAT; 1.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000077184};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 4..277
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 317..461
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
FT REGION 465..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 472..490
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 490 AA; 54336 MW; 4632DC7D5CD50BBB CRC64;
MKKLSFVDKG FLMAETREMP MHVGGVSLYT LPDGVDEHEF MHSLARNVRE ADALLPPFGD
RLKLGRLGIA GNAYWEPDPA LDMDYHVRHS ALPKPGRYRE LFTLVSRLHG TLLERTRPLW
EMHLIEGLKN RQFAVYTKTH HAAVDGARSI HISRSMLSAD PYNVLSESPL SLQSWQRYKD
ALRLGKQAAH TDEELRNAAD MLKSTFDSGT NLFRALKGFT QAWSGRGGEL SLPHLQVPTS
ALNTEVDGAR RFVAQSWPFA RIRAVGSAFD GTFNDAVLAI CAGALRKYLE THAELPEESL
KAMVPVSIRQ AGEVDSGNAV ASISADLATD IVDPAKRIQA IMASVRAGRA FYTDMSPEEI
ELVSMVMQTP SLLLVPMGLI SRMPAYNVAI SNVPGISETM YWNGARMDGS YPLSIVIDGM
AMNITLVTYD QNVDFGIIAC RRSMPHVQRI IDYMEDALVE LEEAAGLSSK AAKRKSKPKR
KRKPRPKAKK
//
