ID A0A162VPI4_DIDRA Unreviewed; 597 AA.
AC A0A162VPI4;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Probable lysosomal cobalamin transporter {ECO:0000256|ARBA:ARBA00017088};
GN ORFNames=EKO05_0002332 {ECO:0000313|EMBL:UPX11741.1}, EKO05_010708
GN {ECO:0000313|EMBL:KAF9702733.1}, ST47_g10329
GN {ECO:0000313|EMBL:KZM18565.1};
OS Didymella rabiei (Chickpea ascochyta blight fungus) (Mycosphaerella
OS rabiei).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Didymellaceae; Ascochyta.
OX NCBI_TaxID=5454 {ECO:0000313|EMBL:KZM18565.1, ECO:0000313|Proteomes:UP000076837};
RN [1] {ECO:0000313|EMBL:KZM18565.1, ECO:0000313|Proteomes:UP000076837}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ArDII {ECO:0000313|EMBL:KZM18565.1,
RC ECO:0000313|Proteomes:UP000076837};
RX PubMed=27091329; DOI=10.1038/srep24638;
RA Verma S., Gazara R.K., Nizam S., Parween S., Chattopadhyay D., Verma P.K.;
RT "Draft genome sequencing and secretome analysis of fungal phytopathogen
RT Ascochyta rabiei provides insight into the necrotrophic effector
RT repertoire.";
RL Sci. Rep. 6:24638-24638(2016).
RN [2] {ECO:0000313|EMBL:KAF9702733.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Me14 {ECO:0000313|EMBL:KAF9702733.1};
RA Syme R.A., Farfan-Caceres L., Lichtenzveig J.;
RL Submitted (DEC-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAF9702733.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Me14 {ECO:0000313|EMBL:KAF9702733.1};
RX PubMed=32345704; DOI=10.1534/g3.120.401265;
RA Shah R.M., Williams A.H., Hane J.K., Lawrence J.A., Farfan-Caceres L.M.,
RA Debler J.W., Oliver R.P., Lee R.C.;
RT "Reference Genome Assembly for Australian Ascochyta rabiei Isolate
RT ArME14.";
RL G3 (Bethesda) 10:2131-2140(2020).
RN [4] {ECO:0000313|EMBL:KAF9702733.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Me14 {ECO:0000313|EMBL:KAF9702733.1};
RA Shah R.M., Williams A.H., Hane J.K., Lawrence J.A., Farfan-Caceres L.M.,
RA Debler J.W., Oliver R.P., Lee R.C.;
RL Submitted (MAY-2020) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:UPX11741.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Me14 {ECO:0000313|EMBL:UPX11741.1};
RA Mobegi F.M., Debler J.W.D., Lee R.C.L.;
RL Submitted (MAR-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable lysosomal cobalamin transporter. Required to export
CC cobalamin from lysosomes allowing its conversion to cofactors.
CC {ECO:0000256|ARBA:ARBA00025515}.
CC -!- SUBCELLULAR LOCATION: Lysosome membrane
CC {ECO:0000256|ARBA:ARBA00004155}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004155}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the LIMR family. LMBRD1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009901}.
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DR EMBL; RYYQ01000023; KAF9702733.1; -; Genomic_DNA.
DR EMBL; JYNV01000326; KZM18565.1; -; Genomic_DNA.
DR EMBL; CP095290; UPX11741.1; -; Genomic_DNA.
DR STRING; 5454.A0A162VPI4; -.
DR OrthoDB; 8533at2759; -.
DR Proteomes; UP000076837; Unassembled WGS sequence.
DR Proteomes; UP000617380; Chromosome 3.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR InterPro; IPR006876; LMBR1-like_membr_prot.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR16130:SF2; LYSOSOMAL COBALAMIN TRANSPORT ESCORT PROTEIN LMBD1; 1.
DR PANTHER; PTHR16130; LYSOSOMAL COBALAMIN TRANSPORTER-RELATED; 1.
DR Pfam; PF04791; LMBR1; 1.
DR SUPFAM; SSF103473; MFS general substrate transporter; 1.
PE 3: Inferred from homology;
KW Cobalamin {ECO:0000256|ARBA:ARBA00022628};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000076837};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448}.
FT TRANSMEM 7..32
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 38..66
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 95..118
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 138..162
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 191..210
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 315..337
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 379..407
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 419..443
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 509..534
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 575..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 597 AA; 66077 MW; 1E522A546B854A15 CRC64;
MALIQTSLIW VAYAVAIGIL LAIASVFVFV YQTPRERAAS ATIVCIFTTL ALLATVLLIP
VDVALVSSTS RSSLGRKKDW ATPEKVHDIT HTLQIVYYLL YSLDALLCLL VVPFTYFYNE
EYDDDAAEHG EQTVGQRILG ALKYTIAFLL FIVIIFLVGF FVPFAKQAKG DKGLDLDFFK
HLLAENHGER ALSFALGLLM TIGTILFVLY TGAGMALLPV AMIKSAPSIS APTLAANTAS
QLEANRERQR QLEGRAEGRQ NGLDARDRRE LEALNREERT LIRRERLAAE SSGEDRHWIV
KTWIKIEAIF RPLKLVGGLV LMLFALFIFA SMLITGIDKA KNSICGAHCG YILGHINIFQ
PLNWILVKSS KVFPIDYVLF LLLVLFLFSA SVVGIATAGI RFLWLTIFKI RKGHTSPQAL
LMATVLLTLI VLAINYSVAM VVAPQYATFG PQTYCDLPVN HLGDQPDCSE HKEAVKACSE
LADNPVAKDV CTPSVLSTFI NRVTINFPFF GVVLFWAQFA FLGVYLIVFL TTLFKVPQLD
QDQLDRDLEE DEEEGLLAST GRRFGATWQD ITGRSKNTYG ATESRNGGRS GEGEGLL
//
