ID A0A163BTF5_9FLAO Unreviewed; 474 AA.
AC A0A163BTF5;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:KZS41741.1};
GN ORFNames=AWE51_20310 {ECO:0000313|EMBL:KZS41741.1};
OS Aquimarina aggregata.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Aquimarina.
OX NCBI_TaxID=1642818 {ECO:0000313|EMBL:KZS41741.1, ECO:0000313|Proteomes:UP000076715};
RN [1] {ECO:0000313|EMBL:KZS41741.1, ECO:0000313|Proteomes:UP000076715}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RZW4-3-2 {ECO:0000313|EMBL:KZS41741.1,
RC ECO:0000313|Proteomes:UP000076715};
RA Wang Y.;
RT "The draft genome sequence of Aquimarina sp. RZW4-3-2.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZS41741.1}.
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DR EMBL; LQRT01000003; KZS41741.1; -; Genomic_DNA.
DR RefSeq; WP_066311368.1; NZ_LQRT01000003.1.
DR AlphaFoldDB; A0A163BTF5; -.
DR STRING; 1642818.AWE51_20310; -.
DR OrthoDB; 9806009at2; -.
DR Proteomes; UP000076715; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR015237; Alpha-amylase_C_pro.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF09154; Alpha-amy_C_pro; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 2.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000076715}.
FT DOMAIN 57..428
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 244
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 268
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 157
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 400
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 474 AA; 53077 MW; 4218AA415E4255C1 CRC64;
MKKIINISTK TILLLGGLVF GLTGCSDDDD SANTTETIVT SQSLKMDLNQ FKREDAGIFM
QAFYWDVEPR GAWYDEVSKS IEDWAASGID RIWLAPPGKA QSGGFSMGYD PCDYFDLGEF
DQHGTVETRF GSRQELENLI QKSHDNGLEV IADIVLNHNS GGQLEANPNT GTDTFTKFDP
ASGLFPRTYD DFYPDETGAT DESVFGGFPD LNHTKENVQN WLWKNENSVA KFYKSIGFDG
WRFDFVKGFA PKWVTAWNEE VGGYSVGENF DGNEQVLKDW VDASGSPAFD FACFYRLDET
LDRNKDLTAL GDAGNMLRKL DPAMAVTFTA NHDTEKDENP NNRISYGNKL LAYSYILTHD
GYPTIFYLDY EAFKGQLKTL MEINSTLAVG DIEILEASKD EYIMKRGGNG TNPGLILYMN
ISKQPKERVV KTNWINKKLV DYTDNTRLTP TTDAEGNTTI YAPAGGYAIW SITK
//
