ID   A0A164VFJ5_9CRUS        Unreviewed;      1070 AA.
AC   A0A164VFJ5;
DT   06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT   06-JUL-2016, sequence version 1.
DT   24-JAN-2024, entry version 18.
DE   RecName: Full=Insulin-degrading enzyme {ECO:0008006|Google:ProtNLM};
GN   ORFNames=APZ42_022325 {ECO:0000313|EMBL:KZS12273.1};
OS   Daphnia magna.
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC   Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX   NCBI_TaxID=35525 {ECO:0000313|EMBL:KZS12273.1, ECO:0000313|Proteomes:UP000076858};
RN   [1] {ECO:0000313|EMBL:KZS12273.1, ECO:0000313|Proteomes:UP000076858}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Xinb3 {ECO:0000313|EMBL:KZS12273.1,
RC   ECO:0000313|Proteomes:UP000076858};
RC   TISSUE=Complete organism {ECO:0000313|EMBL:KZS12273.1};
RA   Gilbert D.G., Choi J.-H., Mockaitis K., Colbourne J., Pfrender M.;
RT   "EvidentialGene: Evidence-directed Construction of Genes on Genomes.";
RL   Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KZS12273.1}.
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DR   EMBL; LRGB01001361; KZS12273.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A164VFJ5; -.
DR   STRING; 35525.A0A164VFJ5; -.
DR   EnsemblMetazoa; XM_032933124.2; XP_032789015.2; LOC116926288.
DR   OrthoDB; 129328at2759; -.
DR   Proteomes; UP000076858; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000076858};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          120..256
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          283..458
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          467..746
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          750..933
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   1070 AA;  122662 MW;  942AF6E40196638B CRC64;
     MILNSNRQTC RCTSCLSQTT ANEDFKSHQK IVVHLKILRN FKPYCFLTVD IVNFARISCK
     VNLRSKILFG NLTLLTRQSS SSPIVKMDGN HSYKRWDNIV KSEQDKREYR GLMLKNEMKI
     LLISDPSTDK SAAAMEVNVG HMCDPHDLPG LAHFCEHMLF LGTEKYPVEN EYPRFLSEHG
     GSSNAFTASD HTNYYFDVVP LQLSAALDRF AQFFLTPLFT ESATDREVNA VDSEHVKNIP
     SDAWRLSQLD KSTSNPNHPY SKFGTGNKDT LDTIPKERGI QVREELLKFH KKWYSANLMS
     LVVLGQESLD ELEKLCVGLF SEVENKNVES PEWKEHPFGP ENLQVKGLVV PVKDIRNLNI
     TFPVPDMREH YETQPERYLS HLIGHEGPGS LLSELKSRGW VNSLMAGESS GAKGFAFFGI
     NVDLTEDGIE HVDDIVTLAF QYLNMLRKLE PQKWVFDELE GLSRVQFRFK DKEKPQSYVC
     SLASKLQYYP MEEVISGDYS FKEWKPDLVT SVLDMLTPEN IRIAVIGKKF ESVADSKEVW
     YGTSYKLEKI DSKDIEAWKN AGLSEKLHMP HRNEFIPEKL DLVTREEPQP WPIILKNSQL
     SRVWFKQDTE FLLPKAVVYI EMFSPIAYLD PLRCSQVCLL ASLFHDALNE FTYAAEVAGL
     GYALQSTKYG LQLSLKGYND KLPTLLQKLI EKLTTFTVDP QRFRILKESY VRALQNFRAE
     QPYQHATYHT NMLLAERAWS KTDLLNSTDD LTVESLQSFI PFLFSQLHLE FLFHGNLTKQ
     QAMDMVDTVE SGLKTHFATK PLLPCQLIRD REVQMNNGAN FLYCADNDVH ATHCVETYLQ
     LGLEDKRSNM LLELAMQILK EPCFNVLRTQ EQLGYIVFSG VRRAHGVQGL RFIVQSEKPP
     TYVDGRIEAF LHSMEQTLKD MSAEEYERHK TALAVRRQEK PKQLSHRAVR YWSEITTGQY
     FFDRDDMEVE ELMQITHQEL LDFFASYVFH QSPLRRKMAV HILASNVSQE KAEPVVHTNG
     GVTLSQPPPQ IRETELVEDV AAFKKSMPLF PLATPIDKNS VLAASAKAKL
//