ID A0A165A0X6_DAUCS Unreviewed; 689 AA.
AC A0A165A0X6;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=ATP-dependent DNA helicase 2 subunit KU80 {ECO:0000256|PIRNR:PIRNR016570};
DE EC=3.6.4.12 {ECO:0000256|PIRNR:PIRNR016570};
GN ORFNames=DCAR_015907 {ECO:0000313|EMBL:KZM96731.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZM96731.1, ECO:0000313|Proteomes:UP000077755};
RN [1] {ECO:0000313|EMBL:KZM96731.1, ECO:0000313|Proteomes:UP000077755}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DH1 {ECO:0000313|Proteomes:UP000077755};
RC TISSUE=Leaf {ECO:0000313|EMBL:KZM96731.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- FUNCTION: Single-stranded DNA-dependent ATP-dependent helicase.
CC {ECO:0000256|PIRNR:PIRNR016570}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|PIRNR:PIRNR016570};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|PIRNR:PIRNR016570}.
CC -!- SIMILARITY: Belongs to the ku80 family. {ECO:0000256|ARBA:ARBA00007726,
CC ECO:0000256|PIRNR:PIRNR016570}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZM96731.1}.
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DR EMBL; LNRQ01000004; KZM96731.1; -; Genomic_DNA.
DR RefSeq; XP_017243553.1; XM_017388064.1.
DR AlphaFoldDB; A0A165A0X6; -.
DR STRING; 79200.A0A165A0X6; -.
DR EnsemblPlants; KZM96731; KZM96731; DCAR_015907.
DR GeneID; 108215567; -.
DR Gramene; KZM96731; KZM96731; DCAR_015907.
DR KEGG; dcr:108215567; -.
DR OMA; WAMQYVW; -.
DR OrthoDB; 5884at2759; -.
DR Proteomes; UP000077755; Chromosome 4.
DR GO; GO:0043564; C:Ku70:Ku80 complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0042162; F:telomeric DNA binding; IEA:InterPro.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006303; P:double-strand break repair via nonhomologous end joining; IEA:InterPro.
DR GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR CDD; cd00873; KU80; 1.
DR Gene3D; 1.10.1600.10; -; 1.
DR Gene3D; 2.40.290.10; -; 1.
DR Gene3D; 1.25.40.240; Ku, C-terminal domain; 1.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR006164; Ku70/Ku80_beta-barrel_dom.
DR InterPro; IPR024193; Ku80.
DR InterPro; IPR005160; Ku_C.
DR InterPro; IPR036494; Ku_C_sf.
DR InterPro; IPR005161; Ku_N.
DR InterPro; IPR014893; Ku_PK_bind.
DR InterPro; IPR016194; SPOC-like_C_dom_sf.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR12604; KU AUTOANTIGEN DNA HELICASE; 1.
DR PANTHER; PTHR12604:SF4; X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5; 1.
DR Pfam; PF02735; Ku; 1.
DR Pfam; PF03730; Ku_C; 1.
DR Pfam; PF03731; Ku_N; 1.
DR Pfam; PF08785; Ku_PK_bind; 1.
DR PIRSF; PIRSF016570; Ku80; 1.
DR SMART; SM00559; Ku78; 1.
DR SUPFAM; SSF101420; C-terminal domain of Ku80; 1.
DR SUPFAM; SSF100939; SPOC domain-like; 1.
DR SUPFAM; SSF53300; vWA-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR016570};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|PIRNR:PIRNR016570};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172,
KW ECO:0000256|PIRNR:PIRNR016570};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|PIRNR:PIRNR016570};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR016570};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PIRNR:PIRNR016570};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR016570};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR016570};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR016570};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755}.
FT DOMAIN 278..419
FT /note="Ku"
FT /evidence="ECO:0000259|SMART:SM00559"
FT REGION 522..542
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 689 AA; 77668 MW; 4E7EA2B360FB37F8 CRC64;
MARNKEALLL LIDVGPSMHT VLPEVEKVCS MLVQKKLIFG KYDDVGVVIF GTEDTDNELT
NEVGGYEHVV VLNHLKVVDG DLIKVLEQLP RGTVDGDFLD AIVVGMDMLI KKFGQTIKGK
KRLCLITDAT HPIKDPFEGT KQDQVSTIAE QMFAHGMKME CIVYRGQQDM DGNTSVIEEN
DILLDIISKM TSSRKVHVEN STSLLGALRT RNISPVTIYR GELELSSEVK VLVWVYKKTS
EEKFPTLKKY SDKAPETDKL ATHEVKVEFE YKKVDDPSKV VPPEQRIKGY RYGPQVVPIS
SAEWEAVKFK PEKGVKLLGF TDASNIMRHY YMKDVNVVIA QPGNMKAALA VSALARGMKE
MNKVAIVRCV WRQGQANVVI GVLSPNISER DDVPDSFYFN VVPFAEDVRE FQFPSFSNLP
SSMLPNNEQQ EAADNLVKML ELSPTGKAEC LQPDFTPNPV LERFYRYLEL KSRHLDAAVP
PLDDTLKKIT EPDSDLLSHS KPVIDGFRKL FELKENPKLK KSSRRLLREK PSGSNEERDD
PGNIVDAEAV NFIEHASSFK VEKIGESTPV QDFESMMLRR DGADWVSKAI ADMKSIIMQL
VTDSFEGDNF QKVLECLIAL RKGCILEQEP KHFNEFLLKL CKFCTEKDLA SFNEFLASKN
VLLISKSEAE ESEITEDEAK IFLVKAEQK
//
