ID A0A165A1M2_DAUCS Unreviewed; 380 AA.
AC A0A165A1M2;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Fe2OG dioxygenase domain-containing protein {ECO:0000259|PROSITE:PS51471};
GN ORFNames=DCAR_015874 {ECO:0000313|EMBL:KZM96764.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZM96764.1};
RN [1] {ECO:0000313|EMBL:KZM96764.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaf {ECO:0000313|EMBL:KZM96764.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 2-oxoglutarate + gibberellin A12 + H(+) + 3 O2 = 3 CO2 +
CC gibberellin A9 + 2 H2O + 2 succinate; Xref=Rhea:RHEA:60772,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:58627, ChEBI:CHEBI:73255;
CC Evidence={ECO:0000256|ARBA:ARBA00043814};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:60773;
CC Evidence={ECO:0000256|ARBA:ARBA00043814};
CC -!- COFACTOR:
CC Name=L-ascorbate; Xref=ChEBI:CHEBI:38290;
CC Evidence={ECO:0000256|ARBA:ARBA00001961};
CC -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC family. GA20OX subfamily. {ECO:0000256|ARBA:ARBA00043997}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZM96764.1}.
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DR EMBL; LNRQ01000004; KZM96764.1; -; Genomic_DNA.
DR RefSeq; XP_017246239.1; XM_017390750.1.
DR AlphaFoldDB; A0A165A1M2; -.
DR STRING; 79200.A0A165A1M2; -.
DR EnsemblPlants; KZM96764; KZM96764; DCAR_015874.
DR GeneID; 108217856; -.
DR Gramene; KZM96764; KZM96764; DCAR_015874.
DR KEGG; dcr:108217856; -.
DR OMA; DEYENFG; -.
DR OrthoDB; 1057251at2759; -.
DR Proteomes; UP000077755; Chromosome 4.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:UniProt.
DR InterPro; IPR026992; DIOX_N.
DR InterPro; IPR044861; IPNS-like_FE2OG_OXY.
DR InterPro; IPR027443; IPNS-like_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR PANTHER; PTHR47990; 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED; 1.
DR PANTHER; PTHR47990:SF53; GIBBERELLIN 20 OXIDASE 1; 1.
DR Pfam; PF03171; 2OG-FeII_Oxy; 1.
DR Pfam; PF14226; DIOX_N; 1.
DR PRINTS; PR00682; IPNSYNTHASE.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU003682};
KW Metal-binding {ECO:0000256|RuleBase:RU003682};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003682};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755}.
FT DOMAIN 222..322
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
SQ SEQUENCE 380 AA; 43216 MW; DAE397CC002B491F CRC64;
MAASQSSRPL HFQHSYEDNN HHSQVVFDAS IIQKETTIPK EFIWPDHEKP SLNTQELKVP
LIDLGAFLSG DPSAAKESSI LIGQACEEHG FFMVVNHGVD TNLISDAHKF MNLFFDLPLY
VKQKAQRKLG ENFGYASSFT GRFASKLPWK ETFSFQYCAD ERMSSTTVED YFTNKFGPDF
AKPGKVYQDY SHAMSTLSLK IMGLLEISLG VSRSHFKNLF EDHDSIVRLN YYPPCQKPEL
TLGTGPHSDP TSLTILHQDH VAGLEVLVDD QWRCILPNTS AFVINIGDTL MAMTNGRYKS
CLHRAVVNNE TARKSIAFFL CPRKDKVVIP PAELVDQKNP RSYPDFTWAE LLQFTQMHYR
ADMNTLDAFS TWLLTKNNRE
//
