ID A0A166CKS9_DAUCS Unreviewed; 594 AA.
AC A0A166CKS9;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE SubName: Full=9-cis-epoxycarotenoid dioxygenase {ECO:0000313|EMBL:KZN04028.1};
GN Name=NCED-7 {ECO:0000313|EMBL:KZN04028.1};
GN ORFNames=DCAR_004826 {ECO:0000313|EMBL:KZN04028.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZN04028.1};
RN [1] {ECO:0000313|EMBL:KZN04028.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaf {ECO:0000313|EMBL:KZN04028.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC Evidence={ECO:0000256|PIRSR:PIRSR604294-1};
CC Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR604294-1};
CC -!- SIMILARITY: Belongs to the carotenoid oxygenase family.
CC {ECO:0000256|ARBA:ARBA00006787}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZN04028.1}.
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DR EMBL; LNRQ01000002; KZN04028.1; -; Genomic_DNA.
DR RefSeq; XP_017234232.1; XM_017378743.1.
DR AlphaFoldDB; A0A166CKS9; -.
DR STRING; 79200.A0A166CKS9; -.
DR EnsemblPlants; KZN04028; KZN04028; DCAR_004826.
DR GeneID; 108208230; -.
DR Gramene; KZN04028; KZN04028; DCAR_004826.
DR KEGG; dcr:108208230; -.
DR OMA; DMIWVDS; -.
DR OrthoDB; 318119at2759; -.
DR Proteomes; UP000077755; Chromosome 2.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016702; F:oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen; IEA:InterPro.
DR InterPro; IPR004294; Carotenoid_Oase.
DR PANTHER; PTHR10543:SF123; 9-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATED; 1.
DR PANTHER; PTHR10543; BETA-CAROTENE DIOXYGENASE; 1.
DR Pfam; PF03055; RPE65; 1.
PE 3: Inferred from homology;
KW Dioxygenase {ECO:0000256|ARBA:ARBA00022964, ECO:0000313|EMBL:KZN04028.1};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRSR:PIRSR604294-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR604294-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00022964,
KW ECO:0000313|EMBL:KZN04028.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755}.
FT REGION 21..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 294
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 343
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 408
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
FT BINDING 581
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR604294-1"
SQ SEQUENCE 594 AA; 66156 MW; 558041EDA7AD9FD9 CRC64;
MASCSSTTTS SFHKTHVISS SCSSSSMKND RTAQSFQSRT MKPCSRKANV VQSALQSPSI
TFPKHPCKLP EITKQDKTMP QKWNLFQKVA ATALDMAENA LLSFESKTPL AKTADPAVQM
AGNFAPVPEQ PVRHNLPVCG TIPDCISGVY VRNGANPLFE PTAGHHLFDG DGMIHAVTIN
GNSVSYACRF TETERLVQER VLGRPVFPKA IGELHGHSGI ARLALFYARG LFGLVDQNHG
IGVANAGLVY FNGRLLAMSE DDLPYHVRVK TSGDLETIGR FDFDSELQST MIAHPKLDPV
SGELFALSYD VIKKPYLKYF RFSEDGKKSE DVEIPLEIPT MTHDFAITEN FVVIPDQQVV
FKLEEMIKGG SPVMYEKNKM SRFGVLPKYA KDSSEIIWVE SPETFCFHLW NAWEEPESDE
VVIIGSCMTP PDSIFNESDE SLESILSEIR LNLKTGKSTR RAIMKPSEQV NIEVGMVNRK
KLGRKTRYAY LAIAEPWPKV SGIAKIDLFT GESQKVFYGD KRYGGEPFFL PSESDKEDEG
YIMAFVHDEQ TWKSELQVIN AATMKLEATV KLPSRVPYGF HGTFISSEEL AQQV
//
