ID A0A166EUZ2_DAUCS Unreviewed; 213 AA.
AC A0A166EUZ2;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=rRNA N-glycosylase {ECO:0000256|RuleBase:RU004915};
DE EC=3.2.2.22 {ECO:0000256|RuleBase:RU004915};
GN ORFNames=DCAR_007849 {ECO:0000313|EMBL:KZN07012.1}, DCAR_007850
GN {ECO:0000313|EMBL:KZN07013.1};
OS Daucus carota subsp. sativus (Carrot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=79200 {ECO:0000313|EMBL:KZN07012.1};
RN [1] {ECO:0000313|EMBL:KZN07012.1, ECO:0000313|Proteomes:UP000077755}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DH1 {ECO:0000313|Proteomes:UP000077755};
RC TISSUE=Leaf {ECO:0000313|EMBL:KZN07012.1};
RX PubMed=27158781; DOI=10.1038/ng.3565;
RA Iorizzo M., Ellison S., Senalik D., Zeng P., Satapoomin P., Huang J.,
RA Bowman M., Iovene M., Sanseverino W., Cavagnaro P., Yildiz M.,
RA Macko-Podgorni A., Moranska E., Grzebelus E., Grzebelus D., Ashrafi H.,
RA Zheng Z., Cheng S., Spooner D., Van Deynze A., Simon P.;
RT "A high-quality carrot genome assembly provides new insights into
RT carotenoid accumulation and asterid genome evolution.";
RL Nat. Genet. 48:657-666(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC adenosine on the 28S rRNA.; EC=3.2.2.22;
CC Evidence={ECO:0000256|RuleBase:RU004915};
CC -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC {ECO:0000256|RuleBase:RU004915}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZN07012.1}.
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DR EMBL; LNRQ01000002; KZN07012.1; -; Genomic_DNA.
DR EMBL; LNRQ01000002; KZN07013.1; -; Genomic_DNA.
DR STRING; 79200.A0A166EUZ2; -.
DR EnsemblPlants; KZN07012; KZN07012; DCAR_007849.
DR EnsemblPlants; KZN07013; KZN07013; DCAR_007850.
DR Gramene; KZN07012; KZN07012; DCAR_007849.
DR Gramene; KZN07013; KZN07013; DCAR_007850.
DR OMA; YRAHFFS; -.
DR Proteomes; UP000077755; Chromosome 2.
DR GO; GO:0030598; F:rRNA N-glycosylase activity; IEA:UniProtKB-EC.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0017148; P:negative regulation of translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.420.10; Ricin (A subunit), domain 1; 1.
DR InterPro; IPR036041; Ribosome-inact_prot_sf.
DR InterPro; IPR017989; Ribosome_inactivat_1/2.
DR InterPro; IPR001574; Ribosome_inactivat_prot.
DR InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR PANTHER; PTHR33453; -; 1.
DR PANTHER; PTHR33453:SF34; DUF6598 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00161; RIP; 1.
DR PRINTS; PR00396; SHIGARICIN.
DR SUPFAM; SSF56371; Ribosome inactivating proteins (RIP); 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|RuleBase:RU004915};
KW Plant defense {ECO:0000256|RuleBase:RU004915};
KW Protein synthesis inhibitor {ECO:0000256|RuleBase:RU004915};
KW Reference proteome {ECO:0000313|Proteomes:UP000077755};
KW Signal {ECO:0000256|SAM:SignalP}; Toxin {ECO:0000256|RuleBase:RU004915}.
FT SIGNAL 1..21
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 22..213
FT /note="rRNA N-glycosylase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5010064583"
SQ SEQUENCE 213 AA; 24087 MW; 48A9B621083C2D63 CRC64;
MAISIIVSRL LFSAVTAACL ASSICSARDK VLLMPPEKIE EKFTFYPILP FDTAQATLKR
YTRFINNVRE EIVSGDTVHG IPRLYNPVKL EESDRYLQVA LFNSDEQRIS LAIDKSDVYI
VGYRTEYEAC FFSDTDGADT SSLFPGITRH QLPFKNGYSG MEEIAGSRRD ISLGMSELDE
CIKHLHDLTD NSSLARCMLI TIQMVAEAVR YRY
//
