ID A0A166QR34_STAPS Unreviewed; 310 AA.
AC A0A166QR34;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE SubName: Full=Lactate dehydrogenase {ECO:0000313|EMBL:PWZ77306.1};
GN ORFNames=DD902_00215 {ECO:0000313|EMBL:PWZ77306.1}, DD924_04095
GN {ECO:0000313|EMBL:PWZ99178.1}, DV961_04120
GN {ECO:0000313|EMBL:REA82925.1}, EGV54_08065
GN {ECO:0000313|EMBL:EGQ4385045.1};
OS Staphylococcus pseudintermedius.
OC Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus; Staphylococcus intermedius group.
OX NCBI_TaxID=283734 {ECO:0000313|EMBL:PWZ77306.1, ECO:0000313|Proteomes:UP000246800};
RN [1] {ECO:0000313|Proteomes:UP000246351, ECO:0000313|Proteomes:UP000246800}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ST525 1 {ECO:0000313|EMBL:PWZ77306.1,
RC ECO:0000313|Proteomes:UP000246800}, and ST71 3
RC {ECO:0000313|EMBL:PWZ99178.1, ECO:0000313|Proteomes:UP000246351};
RX PubMed=29292005; DOI=10.1016/j.vetmic.2017.11.018;
RA Worthing K.A., Abraham S., Coombs G.W., Pang S., Saputra S., Jordan D.,
RA Trott D.J., Norris J.M.;
RT "Clonal diversity and geographic distribution of methicillin-resistant
RT Staphylococcus pseudintermedius from Australian animals: Discovery of novel
RT sequence types.";
RL Vet. Microbiol. 213:58-65(2018).
RN [2] {ECO:0000313|EMBL:REA82925.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ST496-2 {ECO:0000313|EMBL:REA82925.1};
RX PubMed=30173756; DOI=10.1016/j.vetmic.2018.07.021;
RA Worthing K.A., Brown J., Gerber L., Abraham S., Trott D., Norris J.M.;
RT "Methicillin-resistant staphylococci amongst veterinary personnel,
RT personnel-owned pets, patients and the hospital environment of two small
RT animal veterinary hospitals.";
RL Vet. Microbiol. 223:79-85(2018).
RN [3] {ECO:0000313|Proteomes:UP000256409}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ST496-2 {ECO:0000313|Proteomes:UP000256409};
RA Worthing K.A., Brown J., Gerber L., Abraham S., Trott D., Norris J.M.;
RT "Molecular epidemiology of methicillin-resistant staphylococci amongst
RT veterinary personnel, personnel-owned pets, patients and the hospital
RT environment of two companion animal veterinary hospitals.";
RL Vet. Microbiol. 0:0-0(2018).
RN [4] {ECO:0000313|EMBL:EGQ4385045.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=SPSE-18-VL-LA-PA-Ryan-0021 {ECO:0000313|EMBL:EGQ4385045.1};
RG Veterinary Laboratory Investigation and Response Network;
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
CC {ECO:0000256|ARBA:ARBA00006054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWZ77306.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AAXKXX010000011; EGQ4385045.1; -; Genomic_DNA.
DR EMBL; QEIT01000004; PWZ77306.1; -; Genomic_DNA.
DR EMBL; QEIV01000333; PWZ99178.1; -; Genomic_DNA.
DR EMBL; QQPC01000018; REA82925.1; -; Genomic_DNA.
DR RefSeq; WP_014612768.1; NZ_WWPQ01000036.1.
DR AlphaFoldDB; A0A166QR34; -.
DR STRING; 937773.SPSINT_2348; -.
DR eggNOG; COG0039; Bacteria.
DR OMA; SVWSGMN; -.
DR OrthoDB; 9802969at2; -.
DR Proteomes; UP000246351; Unassembled WGS sequence.
DR Proteomes; UP000246800; Unassembled WGS sequence.
DR Proteomes; UP000256409; Unassembled WGS sequence.
DR Proteomes; UP000600220; Unassembled WGS sequence.
DR GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR Gene3D; 3.90.110.10; Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR001557; L-lactate/malate_DH.
DR InterPro; IPR018177; L-lactate_DH_AS.
DR InterPro; IPR022383; Lactate/malate_DH_C.
DR InterPro; IPR001236; Lactate/malate_DH_N.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43128; L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+)); 1.
DR PANTHER; PTHR43128:SF34; L-LACTATE DEHYDROGENASE; 1.
DR Pfam; PF02866; Ldh_1_C; 1.
DR Pfam; PF00056; Ldh_1_N; 1.
DR PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR PRINTS; PR00086; LLDHDRGNASE.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00064; L_LDH; 1.
PE 3: Inferred from homology;
KW NAD {ECO:0000256|PIRSR:PIRSR000102-3};
KW Oxidoreductase {ECO:0000256|RuleBase:RU003369}.
FT DOMAIN 3..140
FT /note="Lactate/malate dehydrogenase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00056"
FT DOMAIN 143..307
FT /note="Lactate/malate dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02866"
FT ACT_SITE 173
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-1"
FT BINDING 8..13
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT BINDING 33
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT BINDING 93
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
FT BINDING 116..118
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000256|PIRSR:PIRSR000102-3"
SQ SEQUENCE 310 AA; 33658 MW; E391A889B9CB5F3F CRC64;
MSKVSIIGAG AVGSTAAYLL SETPWVKEVV LVDVDRDRAQ GQALDMMHGV GVSQAKRVIA
GEYEATQGSD VIIITIGVPE KVGESRLIPL QKNADILKEI VPKMTAYSPN AKIVTVSNPV
DILAYTTYQI SGKAPSDVIG LGTLLDTSRL KYLLSDYFNV SPKSIEATVV GEHGDSQVVL
WRHVRIGGLP LEAFAASQNM MLPDDFKETM AQRVKDTAFD VWKMKGPNCY CVANAIQCLV
EALLSPERRI LPVSNLYTTS TGLTVYISLP SIVSEQGVEQ MLPELLNAEE EAQLNASCAV
MHDYIQQLTL
//
