ID A0A167I3K4_9BURK Unreviewed; 443 AA.
AC A0A167I3K4;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=Isocitrate lyase {ECO:0000256|ARBA:ARBA00017446};
DE EC=4.1.3.1 {ECO:0000256|ARBA:ARBA00012909};
DE AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN ORFNames=LPB072_13285 {ECO:0000313|EMBL:AOW15626.1}, LPB72_11905
GN {ECO:0000313|EMBL:OAD42089.1};
OS Hydrogenophaga crassostreae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Hydrogenophaga.
OX NCBI_TaxID=1763535 {ECO:0000313|EMBL:AOW15626.1, ECO:0000313|Proteomes:UP000185680};
RN [1] {ECO:0000313|EMBL:OAD42089.1, ECO:0000313|Proteomes:UP000185657}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LPB0072 {ECO:0000313|EMBL:OAD42089.1,
RC ECO:0000313|Proteomes:UP000185657};
RA Shin S.-K., Yi H.;
RT "Draft genome sequence of Hydrogenophaga sp. LPB0072.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AOW15626.1, ECO:0000313|Proteomes:UP000185680}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LPB0072 {ECO:0000313|EMBL:AOW15626.1,
RC ECO:0000313|Proteomes:UP000185680};
RA Kim E., Yi H.;
RT "Hydorgenophaga sp. LPB0072 isolated from gastropod.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:36655; EC=4.1.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00023531};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
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DR EMBL; CP017476; AOW15626.1; -; Genomic_DNA.
DR EMBL; LVWD01000013; OAD42089.1; -; Genomic_DNA.
DR RefSeq; WP_066090996.1; NZ_LVWD01000013.1.
DR AlphaFoldDB; A0A167I3K4; -.
DR STRING; 1763535.LPB072_13285; -.
DR KEGG; hyl:LPB072_13285; -.
DR OrthoDB; 8629576at2; -.
DR Proteomes; UP000185657; Unassembled WGS sequence.
DR Proteomes; UP000185680; Chromosome.
DR GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd00377; ICL_PEPM; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR InterPro; IPR039556; ICL/PEPM.
DR InterPro; IPR006254; Isocitrate_lyase.
DR InterPro; IPR018523; Isocitrate_lyase_ph_CS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01346; isocit_lyase; 1.
DR PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR Pfam; PF00463; ICL; 2.
DR PIRSF; PIRSF001362; Isocit_lyase; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00161; ISOCITRATE_LYASE; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:AOW15626.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000185680}.
FT ACT_SITE 203
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT BINDING 99..101
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 165
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
FT BINDING 204..205
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 240
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 325..329
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
FT BINDING 359
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-2"
SQ SEQUENCE 443 AA; 48661 MW; 0337669C25834559 CRC64;
MPQSLNEQLS RQQQIDALEK DWATNPRWKG VKRGYTAADV VRLRGSMNIE HTLAKRGAEK
LWDKINGSAK KGYVNAFGAI SAGQAMQQAK AGLEAVYLSG WQVAADGNTS ETMYPDQSLY
AYDSVPTMVR RINNTFKRAD EIQWGRGVEP GSKEFIDYFL PIVADAEAGF GGVLNAFELM
KNMISAGAAG VHFEDQLAAV KKCGHMGGKV LVPTREACEK LISARFAADV MGVSTIVLAR
TDAEAANLIT SDHDANDKPF LTGERTPEGF YRVKNGLEQS ISRGVAYAPY ADLVWCETGV
PDIGFAREFA QAVHAACPGK LLSYNCSPSF NWKKNLNDKQ IASFQEDLSA LGYKFQFITL
AGIHINWFNT FKFAHAYANG EGMKHYTEMV QEPEFAAREQ GYTFVSHQQE VGAGYFDDVT
TVIQGGSSSV KALTGSTEEE QFH
//
