ID A0A167IXK2_9BURK Unreviewed; 946 AA.
AC A0A167IXK2;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN Name=acnA {ECO:0000313|EMBL:AOW14215.1};
GN ORFNames=LPB072_16595 {ECO:0000313|EMBL:AOW14215.1}, LPB72_04445
GN {ECO:0000313|EMBL:OAD43764.1};
OS Hydrogenophaga crassostreae.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Hydrogenophaga.
OX NCBI_TaxID=1763535 {ECO:0000313|EMBL:AOW14215.1, ECO:0000313|Proteomes:UP000185680};
RN [1] {ECO:0000313|EMBL:OAD43764.1, ECO:0000313|Proteomes:UP000185657}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LPB0072 {ECO:0000313|EMBL:OAD43764.1,
RC ECO:0000313|Proteomes:UP000185657};
RA Shin S.-K., Yi H.;
RT "Draft genome sequence of Hydrogenophaga sp. LPB0072.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AOW14215.1, ECO:0000313|Proteomes:UP000185680}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LPB0072 {ECO:0000313|EMBL:AOW14215.1,
RC ECO:0000313|Proteomes:UP000185680};
RA Kim E., Yi H.;
RT "Hydorgenophaga sp. LPB0072 isolated from gastropod.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; CP017476; AOW14215.1; -; Genomic_DNA.
DR EMBL; LVWD01000003; OAD43764.1; -; Genomic_DNA.
DR RefSeq; WP_066086238.1; NZ_LVWD01000003.1.
DR AlphaFoldDB; A0A167IXK2; -.
DR STRING; 1763535.LPB072_16595; -.
DR KEGG; hyl:LPB072_16595; -.
DR OrthoDB; 9764318at2; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000185657; Unassembled WGS sequence.
DR Proteomes; UP000185680; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000185680}.
FT DOMAIN 62..607
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 740..872
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 946 AA; 101645 MW; E437A2AA31159724 CRC64;
MAPKSFAHPF AKTVKPFKTA SGKSGRLYSL PALAKQFPNV KRLPHSLRIV LESVVRHCDG
EKVTEAHVAQ LANWKPKAKR SAEVPFTVAR VVLQDFTGVP LLADLAAMRS TAERLGQDAK
KIEPLVPVDL VVDHSIMIDY FGQKNAIDLN MKLEFQRNQE RYEFLKWGMQ AFDTFGVVPP
GFGIVHQVNL EYLARGVHMK GGLYYPDTLV GTDSHTTMIN GIGVVAWGVG GIEAEAAMLG
QPVYILTPDV VGFEMTGQLR EGVTATDLVL TVTEILRQHK VVGKFVEFFG EGTASLALPD
RATIANMAPE YGATMGFFPV DERTLDYFRG TGRSKAEIEA FEAYFRAQGM FGVPKAGEID
YSQVVKLDLG KVTPSLAGPK RPQDRIELGQ VADQFRSLFS KPNAENGFNQ DAEHLSLRVP
VSPGGGHSAV GKPADTPAGA SRSVAEMVSN RPVLAAKSKH AGRSLTVGHG DVLIAAITSC
TNTSNPSVLL AAGLLAKKAV EAGLKVEPHI KTSLAPGSRI VTEYLTETGL LPYLEKLGFA
LAGYGCTTCI GNAGDLTPEL NQAINDNQLV CSAVLSGNRN FEARIHPNIK ANFLASPPLV
VAYAIAGTVL TDLMTDPVGV GKGGKPVYLG DIWPSSDEIH ALLKYAMKGK AYRSNYELVK
SEPGKLWGKI KGVSGNAYTW PDSTYIAEPP FFDGFAVAEP AAEKPAVLGA GVMALFGDSI
TTDHISPAGS IKESSPAGEW LKSRGVMKAD FNSYGARRGN HDVMMRGTFA NVRIKNLMLP
AGPDGSREEG GFTLFEGKGA KVAIFDAAQR YMAAGRPTVV FAGEEYGTGS SRDWAAKGTQ
LLGIRAVIAR SFERIHRSNL VGMGVLPLQF KAGESWETLG LKGDESVDVF PHPKLLPQSK
AKLVITRANG ETVETTVTLR IDTPVEVSYY QAGGILPYVL RQLLSA
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